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. 2025 Feb;17(1):450-461.
doi: 10.1007/s12602-023-10168-1. Epub 2023 Oct 4.

An In Vitro Study of the Impacts of Sweet Potato Chips with Potentially Probiotic Levilactobacillus brevis and Lactiplantibacillus plantarum on Human Intestinal Microbiota : Impacts of potato chips with probiotics on intestinal microbiota

Tarsila Gonçalves Feitoza  1 , Bárbara de Lima Ponciano Costa  1 , Karoliny Brito Sampaio  2 , Marcos Dos Santos Lima  3 , Estefânia Fernandes Garcia  2 , Thatyane Mariano Rodrigues de Albuquerque  1 , Evandro Leite de Souza  4 , Noádia Priscila Araújo Rodrigues  2
Affiliations

An In Vitro Study of the Impacts of Sweet Potato Chips with Potentially Probiotic Levilactobacillus brevis and Lactiplantibacillus plantarum on Human Intestinal Microbiota : Impacts of potato chips with probiotics on intestinal microbiota

Tarsila Gonçalves Feitoza et al. Probiotics Antimicrob Proteins. 2025 Feb.

Abstract

This study formulated sweet potato chips with powdered potentially probiotic Levilactobacillus brevis (SPLB) and Lactiplantibacillus plantarum (SPLP) and evaluated their impacts on human intestinal microbiota during 48 h of in vitro colonic fermentation. L. brevis and L. plantarum kept high viable cell counts (> 6 log CFU/g) on sweet potato chips after freeze-drying and during 60 days of storage. SPLB and SPLP had satisfactory quality parameters during 60 days of storage. SPLB and SPLP increased the relative abundance of Lactobacillus ssp./Enterococcus spp. (3.84-10.22%) and Bifidobacterium spp. (3.25-12.45%) and decreased the relative abundance of Bacteroides spp./Prevotella spp. (8.56-2.16%), Clostridium histolyticum (8.23-2.33%), and Eubacterium rectale/Clostridium coccoides (8.07-1.33%) during 48 h of in vitro colonic fermentation. SPLB and SPLP achieved high positive prebiotic indexes (> 8.24), decreased pH values and sugar contents, and increased lactic acid and short-chain fatty acid production, proving selective stimulatory effects on beneficial bacterial groups forming the intestinal microbiota. The results showed that SPLB and SPLP have good stability and high viable cell counts of L. brevis and L. plantarum when stored under room temperature and caused positive impacts on human intestinal microbiota, making them potentially probiotic non-dairy snack options.

Keywords: Lactobacillus; Colonic fermentation; Prebiotic effects; Probiotics; Sweet potato chips.

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Conflict of interest statement

Declarations. Ethical Statement: Ethical approval for the involvement of human subjects in this study was granted by an Ethics Committee for Research with Human Beings (Federal University of Paraíba, João Pessoa, PB, Brazil) with a register number CAAE: 69,561,023.0.0000.5188. Competing Interests: The authors declare no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

References

    1. Misra S, Pandey P, Mishra HN (2021) Novel approaches for co-encapsulation of probiotic bacteria with bioactive compounds, their health benefits and functional food product development: A review. Trends Food Sci Technol 109:340–351. https://doi.org/10.1016/j.tifs.2021.01.039 - DOI
    1. FAO/WHO - Food and Agriculture Organization of the United Nations (2006) Probiotics in food: Health and nutritional properties and guidelines for evaluation. FAO and Nutrition Paper. World Health Organization
    1. Sanders ME, Merenstein DJ, Reid G, Gibson GR, Rastall RA (2019) Probiotics and prebiotics in intestinal health and disease: from biology to the clinic. Nat Rev Gastroenterol Hepatol 16:605–616. https://doi.org/10.1038/s41575-019-0173-3 - DOI - PubMed
    1. Naseri K, Saadati S, Yari Z et al (2022) Beneficial effects of probiotic and synbiotic supplementation on some cardiovascular risk factors among individuals with prediabetes and type 2 diabetes mellitus: A grade-assessed systematic review, meta-analysis, and meta-regression of randomized clinical trials. Pharmacol Res 182:106288. https://doi.org/10.1016/j.phrs.2022.106288 - DOI - PubMed
    1. Musazadeh V, Zarezadeh M, Ghalichi F, Ahrabi SS, Jamiliana P, Jamiliana P, Ghoreishi Z (2022) Anti-obesity properties of probiotics; a considerable medical nutrition intervention: Findings from an umbrella meta-analysis. Eur J Pharmacol 928:175069. https://doi.org/10.1016/j.ejphar.2022.175069 - DOI - PubMed

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