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. 2023 Sep 25:31:e20230184.
doi: 10.1590/1678-7757-2023-0184. eCollection 2023.

Investigating the association between dental age and polymorphisms in genes encoding estrogen receptors

Isabela Ribeiro Madalena  1   2   3 , Caio Luiz Bitencourt Reis  4 , Mirian Aiko Nakane Matsumoto  4 , Maria Bernadete Sasso Stuani  4 , Natanael Henrique Ribeiro Mattos  5 , Daniela Silva Barroso de Oliveira  6 , Maria Angélica Hueb de Menezes Oliveira  2 , Liliane Roskamp  5 , Erika Calvano Küchler  2   5 , Flares Baratto-Filho  1   5
Affiliations

Investigating the association between dental age and polymorphisms in genes encoding estrogen receptors

Isabela Ribeiro Madalena et al. J Appl Oral Sci. .

Abstract

Background: Genetic polymorphisms have been shown to influence several physiological traits, including dental and craniofacial characteristics. Understanding the clinical relevance of genetic polymorphisms in dental practice is crucial to personalize treatment plans and improve treatment outcomes.

Objective: to evaluate the association between dental age and genetic polymorphisms in genes encoding estrogen receptors alpha and beta (ESR1 and ESR2, respectively) in a sample of Brazilian children.

Methodology: This retrospective cross-sectional study was performed with children undergoing orthodontic treatment. Patients with syndromes, congenital anomalies, craniofacial deformities, under hormonal or systemic treatment, and with a previous history of facial trauma were excluded. Panoramic radiographs were used to assess dental age according to the Demirjian, Goldstein, and Tanner method. A delta [dental age-chronological age (DA-CA)] was obtained, which shows whether the patient tends to have a normal, delayed (negative values), or advanced (positive values) dental age. DNA isolated from buccal cells was used to genotype four genetic polymorphisms: rs9340799 (A>G) and rs2234693 (C>T), located in ESR1; and rs1256049 (C>T) and rs4986938 (C>T), located in ESR2. A statistical analysis was performed and values of p<0.05 indicated statistical difference.

Results: A total of 79 patients were included, 44 (55.70%) girls and 35 (44.30%) boys. The Demirjian, Goldstein, and Tanner method, in general, overestimated patients' age by 0.75 years. There was no difference in the delta of dental age between the sexes (p>0.05). Genetic polymorphisms in ESR1 and ESR2 were not associated with dental age (p>0.05).

Conclusion: The studied genetic polymorphisms in ESR1 and ESR2 were not associated with dental age in Brazilian children.

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Conflict of interest statement

Conflict of interest

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1. Flowchart of the sample selection process and outcome
Figure 2
Figure 2. Distribution of delta (DA-CA) according to sex. The mean delta for boys was 0.72 (standard deviation=0.90), while the mean delta for girls was 0.77 (standard deviation=0.91)
See this image and copyright information in PMC

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