Genetic mapping of the p47 L.3.2 mutation in Drosophilamelanogaster

Abstract

An EMS-based forward genetic screen was conducted in an apoptotic null background to identify genetic aberrations that contribute to regulation of cell growth in Drosophila melanogaster . The current work maps the genomic location of one of the identified mutants, L.3.2 . Genetic crosses conducted through the Fly-CURE consortium determined that the gene locus for the L.3.2 mutation is p47 on chromosome 2R.

Figure 1.

The Dark ⁸² , p47 ^L.3.2 mutant results in abnormal cellular structure and hair growth: A.) Lateral view of FRT42D, Dark ⁸² mosaic control eye. Eye tissue from control mosaic (FRT42D, Dark ⁸² ) display a slight R>W phenotype as previously identified (Akdemir et al, 2006). The Dark mosaic eyes have a regular eye shape and size. B.) Lateral view of eyes from mosaic FRT42D, Dark ⁸² ,p47 ^L.3.2 . Dark ⁸² , p47 ^L.3.2 mosaic eyes displayed W>R phenotypes. These also depicted a strong rough eye phenotype and overall reduction in eye size. All eye images captured at 40x. C.) A screenshot of the JBrowse genome browser accessed through FlyBase.org, highlighting the region that failed to complement, via crosses to Df(2R)ED1715 (BDSC 8931) and Df(2R)ED1673 (BDSC 9062); 2R:7,395,885.. 7,489,834. Subsequent single allele complementation mapping demonstrated that the allele, p47 ^L.3.2 (Larkin, 2021) failed to complement (red asterisk).