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. 2023 Sep 30;26(3):257-264.
doi: 10.3831/KPI.2023.26.3.257.

Antiarthritic Activity and Inflammatory Mediators Modulation Effect of Traditional Ajmodadi Churna on Arthritis Experimental Model

Vikash Sharma  1 , Shiv Shankar Shukla  2 , Bina Gidwani  2 , Ravindra Kumar Pandey  3
Affiliations

Antiarthritic Activity and Inflammatory Mediators Modulation Effect of Traditional Ajmodadi Churna on Arthritis Experimental Model

Vikash Sharma et al. J Pharmacopuncture. .

Abstract

Objectives: The study was designed to evaluate anti-arthritic activity of Ajmodadi Churna (AC) and its effect on Complete freund's adjuvant (CFA)-induced arthritis in Wistar rats.

Methods: Arthritis was induced by injecting 0.2 mL CFA into sub plantar surface of left hind paw. Test sample AC-1 and AC-2, 200 and 400 mg/kg, respectively was given to the animals for 21 consecutive days. The increase in swelling was observed after induction of arthritis. The paw edema was measured on 0, 3, 7, 14 and 21 day using Vernier calliper after the induction of arthritis. The collected blood samples further used for the estimation of red blood cells (RBC), white blood cells (WBC), erythrocytes sedimentation rate (ESR), and hemoglobin (Hb), using hematology analyzer. Serum concentration of IL-6 and TNF-α were also measured using rat ELISA kits.

Results: Results showed that a significant reduction in paw edema was observed in AC-2 treated rats. The paw edema was restored on day 21 was 4.48 mm for AC-2, which is near to the control group. The arthritis score in treated rats was found to be considerably lower than in the control group i.e. 0.83 for AC-2 and 1.50 for AC-1. A decrease in levels of RBC and hemoglobin were observed in arthritic rats. Inflammation was significantly reduced and serum levels of IL-6 and TNF-α were lowered after treatment with the test drug.

Conclusion: It can be concluded from the study that AC possess significant anti-arthritic activity. Furthermore, this condition was linked to a reduction in abnormal humoral immune responses.

Keywords: Ayurvedic; ELISA; TNF-α; arthritis; collagen; interleukin.

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Conflict of interest statement

CONFLICTS OF INTEREST We declared no conflict of interest.

Figures

Figure 1
Figure 1
Effect of AC on CFA-induced arthritis in rats. Values are expressed as mean ± SD at n = 6, One-way ANOVA followed by Bonferroni test, significantly different at *p < 0.05, **p > 0.01 and ***p < 0.001, compared to the CFA induced.
Figure 2
Figure 2
Morphology of paw after administration of test drug. (A) Normal control group, (B) CFA induced group, (C) Indomethacin group, (D) AC-1 test group, and (E) AC-2 test group.
Figure 3
Figure 3
Effect of test drug on arthritis index score in CFA induced arthritis rats.
Figure 4
Figure 4
Histopathological slides of rat paw after administration of test drug. (A) Normal control group, (B) CFA induced group, (C) Indomethacin group, (D) AC-1 test group, and (E) AC-2 test group.
See this image and copyright information in PMC

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