Fatty acid composition of whole bodies, specific tissues and cell lines of two lepidopteran insects

Abstract

We detail the fatty acid compositions of last larval instars of two lepidopterans, Spodoptera frugiperda and Trichoplusia ni, two tissues from T. ni, a cell line derived from each species and the respective larval and cell culture media. Larval whole-body and specific tissue fatty acid profiles exhibited the major features commonly found in previous lepidopteran analyses, whereas the cell-line fatty acid compositions were substantially different from the compositions of both their growth media and larvae of their respective species. It appears that these cell-line patterns result from increased monoene biosynthesis in response to low levels of exogenous polyunsaturated fatty acids, a commonly observed essential fatty acid deficiency symptom in whole animals.