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. 2023 Sep 1;23(5):16.
doi: 10.1093/jisesa/iead081.

Transcriptome analysis used to identify and characterize odorant binding proteins in Agasicles hygrophila (Coleoptera: Chryspmelidae)

Changhong Dong  1   2 , Cong Huang  2   3 , Xiaoyu Ning  2   4 , Bo Liu  2 , Xi Qiao  2 , Wanqiang Qian  2 , Daohong Zhu  1 , Fanghao Wan  2   3
Affiliations

Transcriptome analysis used to identify and characterize odorant binding proteins in Agasicles hygrophila (Coleoptera: Chryspmelidae)

Changhong Dong et al. J Insect Sci. .

Erratum in

Abstract

The transcriptomes of Agasicles hygrophila eggs and first instar larvae were analyzed to explore the olfactory mechanism of larval behavior. The analysis resulted in 135,359 unigenes and the identification of 38 odorant-binding proteins (OBPs), including 23 Minus-C OBPs, 8 Plus-C OBPs, and 7 Classic OBPs. Further analysis of differentially expressed genes (DEGs) revealed 10 DEG OBPs, with 5 (AhygOBP5, AhygOBP9, AhygOBP12, AhygOBP15 and AhygOBP36) up-regulated in first instar larvae. Verification of expression patterns of these 5 AhygOBPs using qPCR showed that AhygOBP9 and AhygOBP36 were mainly expressed in the adult stage with gradually increasing expression in the larval stage. AhygOBP5, AhygOBP12, and AhygOBP15 were not expressed in eggs and pupae, and their expression in larvae and adults showed no clear pattern. These 5 AhygOBPs may play an olfactory role in larval behavior, providing a basis for further investigation of their specific functions and clarifying the olfactory mechanism of A. hygrophila.

Keywords: Agasicles hygrophila; egg; first instar larvae; odorant-binding protein; transcriptome analysis.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Multiple sequence alignments of AhygOBPs.
Fig. 2.
Fig. 2.
Motif information of AhygOBPs; Rectangles in different colors represent different Motifs, and the sequences represented by different motifs are shown in the legend.
Fig. 3.
Fig. 3.
Phylogenetic tree of OBP genes from A. hygrophila and other coleopteran species (neighbor-joining tree, NJ); Ahyg: Agasicles hygrophila, Agla: Anoplophora glabripennis, Apla: Agrilus planipennis, Dpon: Dendroctonus ponderosae, Tcas: Tribolium castaneum, Rfer: Rhynchophorus ferrugineus.
Fig. 4.
Fig. 4.
Volcano plot of differentially expressed genes in the eggs and first instar larvae of A. hygrophila; The OBP genes up-regulated in first instar larvae were AhygOBP5, AhygOBP9, AhygOBP12, AhygOBP15, and AhygOBP36.
Fig. 5.
Fig. 5.
Relative expression of the 5 AhygOBP genes throughout different developmental stages; E: egg; L1–L3: 1st-3rd instar larvae; P: pupa; F: female; M: male. The internal reference genes were RPS18, and the relative expression levels are expressed as the mean ± SEM of 3 biological replicates. Multiple comparisons were performed using the LSD method, and different letters on the bars indicate significant differences (P < 0.05).
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