Gene distribution and nucleotide sequence organization in the mouse genome

Abstract

Mouse DNA was fractionated by preparative centrifugation in density gradients of Cs2SO4 containing 3,6-bis(acetatomercurimethyl)dioxane (BAMD). The effects of temperature, BAMD/nucleotide molar ratio and solvent on the fractionation were explored. The fractions so obtained were investigated by analytical centrifugation in CsCl density gradient and by hybridization with a number of gene probes. These approaches led to the definition of satisfactory conditions for the rapid fractionation of mouse DNA; to the localization of a number of genes in mouse DNA fractions; and to a better understanding of the mosaic organization of the mouse genome and, more specifically, to a better estimate of both the intermolecular and intramolecular compositional heterogeneity of mouse DNA in the (75-150) X 10(3)-base size range.