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. 2023 Nov 16;12(11):e0049023.
doi: 10.1128/MRA.00490-23. Epub 2023 Oct 9.

Complete reference genome assemblies and annotations of three Escherichia coli MRE162 clones

Affiliations

Complete reference genome assemblies and annotations of three Escherichia coli MRE162 clones

Russell J S Orr et al. Microbiol Resour Announc. .

Abstract

Escherichia coli MRE162 was originally isolated from a toilet pan in 1949 and since been utilized in numerous studies. Here, we sequence, assemble, and annotate clones held at three laboratories providing reference-level assemblies. We show the uniqueness of MRE162 to strains in open databases and make the UK clone publically available.

Keywords: DNA sequencing; Escherichia coli; genome analysis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
Genome map of MRE162 UK clone. Circular representation of the MRE162 UK clone genome. The upper map shows the chromosome, with the lower depicting the plasmid. Genomic features and Cluster of Orthologs Groups (COGs) with corresponding keys are mapped on the genome representation. Genomic features and COGs obtained from PGAP annotation.

References

    1. May KR, Druett HA. 1968. A Microthread technique for studying the viability of Microbes in a simulated airborne state. J Gen Microbiol 51:353–366. doi:10.1099/00221287-51-3-353 - DOI - PubMed
    1. Hood AM. 1974. Open-air factors in enclosed systems. J Hyg (Lond) 72:53–60. doi:10.1017/s0022172400023202 - DOI - PMC - PubMed
    1. Fernandez MO, Thomas RJ, Garton NJ, Hudson A, Haddrell A, Reid JP. 2019. Assessing the airborne survival of bacteria in populations of aerosol droplets with a novel technology. J R Soc Interface 16:20180779. doi:10.1098/rsif.2018.0779 - DOI - PMC - PubMed
    1. Thomas RJ, Webber D, Hopkins R, Frost A, Laws T, Jayasekera PN, Atkins T. 2011. The cell membrane as a major site of damage during Aerosolization of Escherichia coli. Appl Environ Microbiol 77:920–925. doi:10.1128/AEM.01116-10 - DOI - PMC - PubMed
    1. Thomas RJ, Webber D, Sellors W, Collinge A, Frost A, Stagg AJ, Bailey SC, Jayasekera PN, Taylor RR, Eley S, Titball RW. 2008. Characterization and deposition of Respirable Large- and small-particle Bioaerosols. Appl Environ Microbiol 74:6437–6443. doi:10.1128/AEM.01194-08 - DOI - PMC - PubMed

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