Characterization of highly pathogenic clade 2.3.4.4b H5N1 mink influenza viruses

doi:10.1016/j.ebiom.2023.104827

. 2023 Nov:97:104827.

doi: 10.1016/j.ebiom.2023.104827. Epub 2023 Oct 7.

Characterization of highly pathogenic clade 2.3.4.4b H5N1 mink influenza viruses

Tadashi Maemura¹, Lizheng Guan¹, Chunyang Gu¹, Amie Eisfeld¹, Asim Biswas¹, Peter Halfmann¹, Gabriele Neumann¹, Yoshihiro Kawaoka²

Affiliations

¹ Department of Pathobiological Sciences, Influenza Research Institute, School of Veterinary Medicine, University of Wisconsin-Madison, 575 Science Drive, Madison, WI 53711, USA.
² Department of Pathobiological Sciences, Influenza Research Institute, School of Veterinary Medicine, University of Wisconsin-Madison, 575 Science Drive, Madison, WI 53711, USA; Division of Virology, Department of Microbiology and Immunology, International Research Center for Infectious Diseases, The Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; Research Center for Global Viral Diseases, National Center for Global Health and Medicine, Tokyo 162-8655, Japan; The University of Tokyo, Pandemic Preparedness, Infection and Advanced Research (UTOPIA) Center, Tokyo 108-8639, Japan. Electronic address: yoshihiro.kawaoka@wisc.edu.

PMID: 37812908
PMCID: PMC10579283
DOI: 10.1016/j.ebiom.2023.104827

Characterization of highly pathogenic clade 2.3.4.4b H5N1 mink influenza viruses

Tadashi Maemura et al. EBioMedicine. 2023 Nov.

. 2023 Nov:97:104827.

doi: 10.1016/j.ebiom.2023.104827. Epub 2023 Oct 7.

Authors

Tadashi Maemura¹, Lizheng Guan¹, Chunyang Gu¹, Amie Eisfeld¹, Asim Biswas¹, Peter Halfmann¹, Gabriele Neumann¹, Yoshihiro Kawaoka²

Affiliations

¹ Department of Pathobiological Sciences, Influenza Research Institute, School of Veterinary Medicine, University of Wisconsin-Madison, 575 Science Drive, Madison, WI 53711, USA.
² Department of Pathobiological Sciences, Influenza Research Institute, School of Veterinary Medicine, University of Wisconsin-Madison, 575 Science Drive, Madison, WI 53711, USA; Division of Virology, Department of Microbiology and Immunology, International Research Center for Infectious Diseases, The Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; Research Center for Global Viral Diseases, National Center for Global Health and Medicine, Tokyo 162-8655, Japan; The University of Tokyo, Pandemic Preparedness, Infection and Advanced Research (UTOPIA) Center, Tokyo 108-8639, Japan. Electronic address: yoshihiro.kawaoka@wisc.edu.

PMID: 37812908
PMCID: PMC10579283
DOI: 10.1016/j.ebiom.2023.104827

No abstract available

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Conflict of interest statement

Declaration of interests GN and YK are co-founders of FluGen. YK is funded by a Research Program on Emerging and Reemerging Infectious Diseases (JP21fk0108552 and JP21fk0108615), by a Project Promoting Support for Drug Discovery (JP21nf0101632), by the Japan Program for Infectious Diseases Research and Infrastructure (JP23wm0125002), and by the Japan Initiative for World-leading Vaccine Research and Development Centers (JP233fa627001) from the Japan Agency for Medical Research and Development. YK has received unrelated funding support from Daiichi Sankyo Pharmaceutical, Fuji Film (Toyama Chemical), Tauns Laboratories, Shionogi, Otsuka Pharmaceutical, KM Biologics, Kyoritsu Seiyaku, Shinya Corporation, and Fuji Rebio. All other authors declare no competing interests.

Figures

**Fig. 1**
**Virulence and transmissibility of clade 2.3.4.4b mink H5N1 viruses in ferrets.** Ferrets (three per group) were inoculated with 10⁶ plaque-forming units (PFU) of A/mink/Spain/22VIR12774-13_3869-2/2022 (H5N1, mink 3869-2), A/mink/Spain/22VIR12774-14_3869-3/2022 (H5N1, mink 3869-3), or A/Isumi/UT-KK001-01/2018 (H1N1, H1N1pdm) virus. One day later, naïve animals were placed into cages that allowed air flow, but no direct contact between the infected and exposed animals. Nasal swab samples were collected at the indicated timepoints and virus titers were determined by performing plaque assays with MDCK cells. ∗Ferrets met euthanasia criteria. ^†Ferrets were euthanized on Day 10 post-infection or Day 9 post-exposure, respectively, because the virus infection had cleared. The dotted line indicates the detection limit (1.0 log₁₀ PFU/ml).

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References

1. Herfst S., Schrauwen E.J., Linster M., et al. Airborne transmission of influenza A/H5N1 virus between ferrets. Science. 2012;336(6088):1534–1541. - PMC - PubMed
1. Imai M., Watanabe T., Hatta M., et al. Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets. Nature. 2012;486(7403):420–428. - PMC - PubMed
1. Aguero M., Monne I., Sanchez A., et al. Highly pathogenic avian influenza A(H5N1) virus infection in farmed minks, Spain, 2022. Euro Surveill. 2023;28(3) - PMC - PubMed
1. Puryear W., Sawatzki K., Hill N., et al. Highly pathogenic avian influenza A(H5N1) virus outbreak in New England Seals, United States. Emerg Infect Dis. 2023;29(4):786–791. - PMC - PubMed
1. Bryce Warner T., Vendramelli R., Moffat E., et al. Transmission of lethal H5N1 clade 2.3.4.4b avian influenza in ferrets. Res Square. 2023 https://wwwresearchsquarecom/article/rs-2842567/v1

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[1] Herfst S., Schrauwen E.J., Linster M., et al. Airborne transmission of influenza A/H5N1 virus between ferrets. Science. 2012;336(6088):1534–1541. - PMC - PubMed

[2] Herfst S., Schrauwen E.J., Linster M., et al. Airborne transmission of influenza A/H5N1 virus between ferrets. Science. 2012;336(6088):1534–1541. - PMC - PubMed

[3] Imai M., Watanabe T., Hatta M., et al. Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets. Nature. 2012;486(7403):420–428. - PMC - PubMed

[4] Imai M., Watanabe T., Hatta M., et al. Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets. Nature. 2012;486(7403):420–428. - PMC - PubMed

[5] Aguero M., Monne I., Sanchez A., et al. Highly pathogenic avian influenza A(H5N1) virus infection in farmed minks, Spain, 2022. Euro Surveill. 2023;28(3) - PMC - PubMed

[6] Aguero M., Monne I., Sanchez A., et al. Highly pathogenic avian influenza A(H5N1) virus infection in farmed minks, Spain, 2022. Euro Surveill. 2023;28(3) - PMC - PubMed

[7] Puryear W., Sawatzki K., Hill N., et al. Highly pathogenic avian influenza A(H5N1) virus outbreak in New England Seals, United States. Emerg Infect Dis. 2023;29(4):786–791. - PMC - PubMed

[8] Puryear W., Sawatzki K., Hill N., et al. Highly pathogenic avian influenza A(H5N1) virus outbreak in New England Seals, United States. Emerg Infect Dis. 2023;29(4):786–791. - PMC - PubMed

[9] Bryce Warner T., Vendramelli R., Moffat E., et al. Transmission of lethal H5N1 clade 2.3.4.4b avian influenza in ferrets. Res Square. 2023 https://wwwresearchsquarecom/article/rs-2842567/v1

[10] Bryce Warner T., Vendramelli R., Moffat E., et al. Transmission of lethal H5N1 clade 2.3.4.4b avian influenza in ferrets. Res Square. 2023 https://wwwresearchsquarecom/article/rs-2842567/v1

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Characterization of highly pathogenic clade 2.3.4.4b H5N1 mink influenza viruses

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Characterization of highly pathogenic clade 2.3.4.4b H5N1 mink influenza viruses

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