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. 2023 Dec 1;50(12):821-826.
doi: 10.1097/OLQ.0000000000001877. Epub 2023 Oct 12.

Antimicrobial Resistance and Molecular Typing of Neisseria gonorrhoeae Isolates From the Eastern Cape Province in South Africa

Remco P H Peters, Hyunsul Jung  1 , Nireshni Mitchev  2 , Mandisa M Mdingi  3 , Ranjana Gigi, Amir Shroufi  4 , Fernando P Martinez  5 , Colleen Bamford
Affiliations

Antimicrobial Resistance and Molecular Typing of Neisseria gonorrhoeae Isolates From the Eastern Cape Province in South Africa

Remco P H Peters et al. Sex Transm Dis. .

Abstract

Background: There is a paucity of Neisseria gonorrhoeae antimicrobial resistance data from resource-constrained settings because of the lack of diagnostic testing and limited scale of surveillance programs. This study aimed to determine the antimicrobial resistance profile of N. gonorrhoeae in the rural Eastern Cape province of South Africa.

Methods: Specimens for N. gonorrhoeae culture were obtained from men with urethral discharge and women with vaginal discharge attending primary health care facilities. Direct inoculation of the agar plates was performed followed by culture and drug susceptibility testing using the Etest at the laboratory. Whole-genome sequencing of the isolates was performed to identify resistance-determining variants.

Results: One hundred N. gonorrhoeae isolates were obtained. Most strains were nonsusceptible to ciprofloxacin (76%), tetracycline (75%), and penicillin G (72%). The gyrA S91F mutation was present in 68 of 72 ciprofloxacin-resistant isolates (94%), with concurrent parC mutations in 47 of 68 (69%); gyrA I250M was the only mutation in 4 other resistant strains. One azithromycin-resistant isolate was identified with a minimal inhibitory concentration (MIC) of 8.0 mg/L and the 23S rDNA gene mutation C2597T. The median MIC of cefixime was 0.016 mg/L (range, 0.016-0.064 mg/L), and that of ceftriaxone was 0.016 mg/L (range, 0.016 mg/L). Whole-genome sequencing showed 58 sequence types as revealed in N. gonorrhoeae sequence typing for antimicrobial resistance and 70 sequence types in N. gonorrhoeae multiantigen sequence typing.

Conclusions: This study confirmed high rates of N. gonorrhoeae antimicrobial resistance to ciprofloxacin, penicillin G, and tetracycline in our setting. The MICs of cephalosporins are reassuring for ceftriaxone use in syndromic treatment regimens, but the identification of azithromycin resistance warrants further attention.

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Conflict of interest statement

Conflict of Interest and Sources of Funding: None of the authors declare a conflict of interest. This study was funded by the South African Medical Research Council through a grant awarded to the Global Antibiotic Research and Development Partnership (GARDP) under the project “Evaluating antimicrobial resistance of Neisseria gonorrhoeae infection in population groups at high risk of STI acquisition” (SAMRC project code 96150).

References

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