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. 2023 Jul 5;11(10):6140-6150.
doi: 10.1002/fsn3.3550. eCollection 2023 Oct.

Escherichia coli O157:H7 beef carcass contamination and its antibiotic resistance in Awi Zone, Northwest Ethiopia

Aschalew Ayisheshim Tarekegn  1 , Birhan Agimas Mitiku  2 , Yeshwas Ferede Alemu  2
Affiliations

Escherichia coli O157:H7 beef carcass contamination and its antibiotic resistance in Awi Zone, Northwest Ethiopia

Aschalew Ayisheshim Tarekegn et al. Food Sci Nutr. .

Abstract

Escherichia coli O157:H7 is a cause of foodborne disease and global public health issues especially in developing countries like Ethiopia. A cross-sectional study was done from January 2022 to July 2022 in Awi Zone to assess the occurrence and antibiograms of E. coli O157:H7. Abattoirs and butcher shops were selected purposively, whereas a systematic random and purposive sampling technique was employed to select study units in abattoirs and butcher shops, respectively. A total of 248 swab samples were collected, isolated, and confirmed using bacteriological culture, biochemical tests, and latex agglutination tests. Escherichia coli O1157:H7 antibiogram tests were performed using Kirby-Bauer disk diffusion method. Logistic regression was used to analyze and measure the degree of association between the presumed risk factors and E. coli O157:H7 occurrence. The overall occurrence of E. coli O157:H7 was estimated to be 8.87% and a relative higher (11.29%) occurrence of E. coli O157:H7 was recorded at butcher shops when compared to abattoirs (6.45%). All isolates were susceptible to gentamicin followed by chloramphenicol (81.81%). About 81.81% of the isolates were resistant to ampicillin and 77.23% of isolates developed resistance to two and more than two antibiotics (MDR). In conclusion, E. coli O157:H7 was detected in the study area. Thus, educating abattoir and butcher shop workers, and consumers, on hygienic handling practices and safe consumption of meat could eliminate foodborne infection associated with E. coli O157:H7 occurrence.

Keywords: Awi Zone; E. coli O157:H7; abattoir; antibiograms; butcher shop.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

FIGURE 1
FIGURE 1
Locations of the study area (ArcMap output from Ethiopian shape file).
FIGURE 2
FIGURE 2
Percentage‐wise antibiograms of Escherichia coli O157:H7 isolates with antimicrobial agents.
FIGURE 3
FIGURE 3
Monodrug resistance of Escherichia coli O157:H7 isolates to antimicrobial agents.
See this image and copyright information in PMC

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