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. 2024 Jan 31;194(2):612-617.
doi: 10.1093/plphys/kiad538.

Cellulose biosynthesis inhibitor isoxaben causes nutrient-dependent and tissue-specific Arabidopsis phenotypes

Michael Ogden  1   2 , Sarah J Whitcomb  2   3 , Ghazanfar Abbas Khan  4 , Ute Roessner  5 , Rainer Hoefgen  2 , Staffan Persson  1   6
Affiliations

Cellulose biosynthesis inhibitor isoxaben causes nutrient-dependent and tissue-specific Arabidopsis phenotypes

Michael Ogden et al. Plant Physiol. .
No abstract available

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Conflict of interest statement

Conflict of interest statement. None declared.

Figures

Figure 1.
Figure 1.
The phenotypic response to isoxaben is nutrient-dependent. A) Representative Col-0 root phenotypes 7 d after stratification. Seedlings were grown on 1× MS media (M407, PhytoTech Labs) lacking nitrogen, potassium, and phosphorus, modified to contain a final concentration of 1.35 mM phosphate (as KH2PO4) and each indicated nutrient, with 1.5 nM isoxaben (isx, +) or mock (ethanol, −). B) Isoxaben-induced change in root length, quantified from the growth assay in A). Error bars represent means ± Sd (n = 9 to 29). Asterisk denotes significant difference from control (P < 0.001), calculated using 2-sample t test. C) Representative Col-0 root phenotypes 8 d after stratification, grown on the indicated concentrations of standard MS media (M0222, Duchefa) and isoxaben or mock.
Figure 2.
Figure 2.
The phenotypic response to isoxaben is tissue-specific, and the transcriptional response is nutrient-dependent. A) RNA-Seq analysis of Col-0 roots from seedlings grown vertically on modified 1× MS media and harvested at 6 d after stratification. For short-term treatment, seedlings were grown on media containing 6 mM or 60 mM KNO3, and 5 d after stratification, they were shifted to media containing the same concentration of KNO3 with either 2.5 nM isoxaben or mock. Roots were harvested 24 h later on Day 6. For long-term treatment, seedlings were grown on media containing 6 mM or 60 mM KNO3 with 2.5 nM isoxaben or mock, and roots were harvested at 6 d after stratification. RNA was extracted using a commercial kit, libraries were prepared using poly(A) enrichment, and strand-specific 150 bp paired-end sequencing was carried out. Values indicate the number of DEGs showing a specific response to 2.5 nM isoxaben (padj < 0.01, n = 3). See Supplemental Methods for a detailed overview of the experimental setup and data analysis. B) Representative Col-0 shoot phenotypes from seedlings grown on modified 1× MS media containing 60 mM KNO3 and 2.5 nM isoxaben (isx) or mock (ethanol). Seedlings were imaged at the indicated number of days after stratification (d) from 4 independent growth assays. C) Crystalline cellulose quantification from Col-0 seedlings grown on modified 1× MS containing 6 mM or 60 mM KNO3 and 2.5 nM isx or mock (-isx). Tissue was harvested 10 d after stratification and cellulose quantified using a variation of the standard Updegraff method (see Supplemental Methods). Different letters indicate statistically different values within each tissue type (P < 0.05), calculated using 1-way ANOVA followed by Tukey's post hoc test. Error bars represent means ± Sd (n = 5).
See this image and copyright information in PMC

References

    1. Brabham C, Stork J, Barrett M, DeBolt S. Grass cell walls have a role in the inherent tolerance of grasses to the cellulose biosynthesis inhibitor isoxaben. Pest Manag Sci. 2018:74(4):878–884. 10.1002/ps.4779 - DOI - PubMed
    1. Denness L, McKenna JF, Segonzac C, Wormit A, Madhou P, Bennett M, Mansfield J, Zipfel C, Hamann T. Cell wall damage-induced lignin biosynthesis is regulated by a reactive oxygen species- and jasmonic acid-dependent process in Arabidopsis. Plant Physiol. 2011:156(3):1364–1374. 10.1104/pp.111.175737 - DOI - PMC - PubMed
    1. Desprez T, Vernhettes S, Fagard M, Refregier G, Desnos T, Aletti E, Py N, Pelletier S, Hofte H. Resistance against herbicide isoxaben and cellulose deficiency caused by distinct mutations in same cellulose synthase isoform CESA6. Plant Physiol. 2002:128(2):482–490. 10.1104/pp.010822 - DOI - PMC - PubMed
    1. Engelsdorf T, Gigli-Bisceglia N, Veerabagu M, McKenna JF, Vaahtera L, Augstein F, Van der Does D, Zipfel C, Hamann T. The plant cell wall integrity maintenance and immune signaling systems cooperate to control stress responses in Arabidopsis thaliana. Sci Signal. 2018:11:536. 10.1126/scisignal.aao3070 - DOI - PubMed
    1. Gruber BD, Giehl RF, Friedel S, von Wiren N. Plasticity of the Arabidopsis root system under nutrient deficiencies. Plant Physiol. 2013:163(1):161–179. 10.1104/pp.113.218453 - DOI - PMC - PubMed