Quantitative alterations in the metabolism of carbonyl compounds due to diet-induced lipid peroxidation in rats

Abstract

Following the dietary induction of lipid peroxidation in rats (verified by the levels of malonaldehyde and glutathione peroxidase), the urinary carbonyl compounds were followed chromatographically. Through a headspace gas chromatographic procedure, increases of several aldehydes and furan derivatives were noticed. Liquid chromatography of the dinitrophenylhydrazone derivatives of urinary carbonyls provided a more definitive experiment, in which the increased-peroxidation animals could be compared to those maintained on a control diet. Several carbonyl metabolites, identified by mass spectrometry, were elevated following the lipid peroxidation induction.