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. 1986 Oct 31:382:99-106.
doi: 10.1016/s0378-4347(00)83508-2.

High-performance liquid chromatographic determination of N-acetylcysteine in human serum following acetaminophen overdosage

M R Holdiness, L R Morgan Jr, L E Gillen, E F Harrison

High-performance liquid chromatographic determination of N-acetylcysteine in human serum following acetaminophen overdosage

M R Holdiness et al. J Chromatogr. .

Abstract

An analytical method has been developed for the determination of N-acetylcysteine in human serum following acetaminophen overdosage in humans. Serum samples were treated with dithiothreitol and the protein-freed product was derivatized with 2,4-dinitrofluorobenzene. N-Acetylhomocysteine thiolactone was used as an internal standard. Following diethyl ether extraction, the components were separated on a reversed-phase column with retention times of 7.4 and 9.9 min for N-acetylcysteine and internal standard, respectively. Ultraviolet detection at 365 nm was employed and little interference was noted from other serum components. The method has been applied to quantitation of N-acetylcysteine given as treatment for acetaminophen intoxication.

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