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. 2023 Sep 28;12(19):3608.
doi: 10.3390/foods12193608.

Traceability Research on Dendrobium devonianum Based on SWATHtoMRM

Tao Lin  1 , Xinglian Chen  1 , Lijuan Du  1 , Jing Wang  2 , Zhengxu Hu  2 , Long Cheng  3 , Zhenhuan Liu  1 , Hongcheng Liu  1
Affiliations

Traceability Research on Dendrobium devonianum Based on SWATHtoMRM

Tao Lin et al. Foods. .

Abstract

SWATHtoMRM technology was used in this experiment to further identify and trace the sources of Dendrobium devonianum and Dendrobium officinale produced in the same area using TOF and MS-MRM. After the conversion of the R package of SWATHtoMRM, 191 MRM pairs of positive ions and 96 pairs of negative ions were obtained. Dendrobium devonianum and Dendrobium officinale can be separated very well using the PCA and PLS-DA analysis of MRM ion pairs; this shows that there are obvious differences in chemical composition between Dendrobium devonianum and Dendrobium officinale, which clearly proves that the pseudotargeted metabolomics method based on SWATHtoMRM can be used for traceability identification research. A total of 146 characteristic compounds were obtained, with 20 characteristic compounds in Dendrobium devonianum. The enrichment pathways of the characteristic compounds were mainly concentrated in lipids and atherosclerosis, chagas disease, fluid shear stress and atherosclerosis, proteoglycans in cancer, the IL-17 signaling pathway, the sphingolipid signaling pathway, diabetic cardiomyopathy, arginine and proline metabolism, etc., among which the lipid and atherosclerosis pathways were more enriched, and 11 characteristic compounds affected the expression levels of IL-1, TNFα, CD36, IL-1β, etc. These can be used as a reference for research on variety improvement and active substance accumulation in Dendrobium devonianum and Dendrobium officinale.

Keywords: Dendrobium devonianum; Longling area; identification; time-of-flight mass spectrometry.

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Conflict of interest statement

Author Long Cheng was employed by the company SCIEX Analytical Instrument Trading Co., Ltd., which had the role of mass spectrometry detection and data analysis in this study, without any potential conflict. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationship that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Molecular weight distribution of compounds converted using SWATHtoMRM.
Figure 2
Figure 2
Loading plots of PCA of Dendrobium devonianum and Dendrobium officinale (T: Dendrobium officinale, Z: Dendrobium devonianum, QC: QC samples).
Figure 3
Figure 3
PLS-DA score chart and model diagram of Dendrobium devonianum and Dendrobium officinale (T: Dendrobium officinale, red range; Z: Dendrobium devonianum, green range).
Figure 4
Figure 4
PLS-DA cross validation details of Dendrobium devonianum and Dendrobium officinale. (* The Q2 value was 0.96961, which was also the largest).
Figure 5
Figure 5
Scatter plot of 146 characteristic compounds.
Figure 6
Figure 6
Molecular weight distribution of 146 characteristic compounds (the numbers on the bar graph represent the number of characteristic compounds in that molecular weight range).
Figure 7
Figure 7
The VIP scores of Dendrobium devonianum and Dendrobium officinale (T: Dendrobium officinale, Z: Dendrobium devonianum).
Figure 8
Figure 8
A volcano plot of Dendrobium devonianum and Dendrobium officinale.
Figure 9
Figure 9
A heatmap of Dendrobium devonianum (Z) and Dendrobium officinale (T).
Figure 10
Figure 10
Box plot of the five characteristic compounds (T: Dendrobium officinale, red range, Z: Dendrobium devonianum, green range).
Figure 10
Figure 10
Box plot of the five characteristic compounds (T: Dendrobium officinale, red range, Z: Dendrobium devonianum, green range).
Figure 10
Figure 10
Box plot of the five characteristic compounds (T: Dendrobium officinale, red range, Z: Dendrobium devonianum, green range).
Figure 11
Figure 11
Barplot and dotplot of the top 20 KEGG enrichment pathways based on 11 characteristic compounds.
Figure 12
Figure 12
Lipid and atherosclerosis metabolic pathway.
See this image and copyright information in PMC

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