Arbuscular Mycorrhizal Fungi Restored the Saline-Alkali Soil and Promoted the Growth of Peanut Roots

Abstract

Peanut (Arachis hypogaea L.) is an important oil and cash crop. An efficient utilization of saline-alkali soil resources, the development of peanut planting in saline-alkali soil, and obtaining high and stable yield have become urgent needs to ensure peanut production. Arbuscular mycorrhizal fungi (AMF) have been reported to develop the potential productivity of host plants and improve their salt resistance and tolerance. However, there is still limited research on promoting the growth and morphology of peanut roots. Therefore, in this study, seeds of salt-tolerant peanut variety "HY 25" were coated with commercial AMF inoculant before being planted in saline-alkali and normal soils to investigate the effects of AMF on peanut root growth and rhizosphere soil. The results showed that root morphological characteristics were significantly increased by the use of AMF at the podding stage in saline-alkali soil and from the flowering and pegging stage to the maturity stage in normal soil. Of note, the total root volume of peanut inoculated with AMF significantly increased by 31.57% during the podding stage in saline-alkali soil. Meanwhile, AMF significantly increased the phosphatase and invertase activities in the peanut rhizosphere of saline-alkali soil from the flowering stage to maturity stage and soil CAT activity at the maturity stage (41.16~48.82%). In normal soil, soil phosphatase and urease activities were enhanced by AMF at the flowering stage and the podding stage, respectively. AMF also increased the contents of soil organic matter, available phosphorus, and hydrolysable nitrogen, but decreased soil EC in saline-alkali soil. In addition to the significant increase in soil available phosphorus content, AMF had no significant effect on the physical and chemical properties of the soil and other soil nutrients in normal soil. AMF significantly increased pod biomass and yield in saline-alkali soil and normal soil, and improved their agronomic characteristics. In conclusion, peanut seeds coated with AMF improved the root morphological characteristics of peanuts and improved the physical and chemical properties in peanut rhizosphere, especially in saline-alkali soil. The process of rhizosphere soil nutrient transformation was also enhanced. Finally, AMF improved plant agronomic traits to increase the pod yield (16.5~21.9%). This study provides the theoretical basis and technical support for the application of AMF in peanut production in saline-alkali soil.

Keywords: arbuscular mycorrhizal fungi; peanuts; saline–alkali soil; soil enzyme activity; soil nutrient.

Figure 1

Influence of AMF on total root length of peanut in saline–alkali and normal soils. (A): GR saline–alkali soil, (B): LX normal soil. ns indicates non-significance; * indicates significant differences at p ≤ 0.05; ** indicates significant differences at p ≤ 0.01; data were expressed as mean ± SD (n = 3).

Figure 2

Influence of AMF on root surface area of peanut in saline–alkali and normal soils. (A): GR saline–alkali soil, (B): LX normal soil. ns indicates non-significance; ns indicates non-significance; * indicates significant differences at p ≤ 0.05; ** indicates significant differences at p ≤ 0.01; data were expressed as mean ± SD (n = 3).

Figure 3

Influence of AMF on root diagram of peanut in saline–alkali and normal soils. (A): GR saline–alkali soil, (B): LX normal soil. ns indicates non-significance; * indicates significant differences at p ≤ 0.05; data were expressed as mean ± SD (n = 3).

Figure 4

Influence of AMF on root volume of peanut in saline–alkali and normal soils. (A): GR saline–alkali soil, (B): LX normal soil. ns indicates non-significance; * indicates significant differences at p ≤ 0.05; ** indicates significant differences at p ≤ 0.01; data were expressed as mean ± SD (n = 3).

Figure 5

Influence of AMF on soil enzyme activities in peanut rhizosphere in saline–alkali and normal soils. (A,C,E,G): GR saline–alkali soil, (B,D,F,H): LX normal soil. ns indicates non-significance; * indicates significant differences at p ≤ 0.05; ** indicates significant differences at p ≤ 0.01; data were expressed as mean ± SD (n = 3).

Figure 6

Influence of AMF on soil pH, EC and soil organic matters in peanut rhizosphere at mature stage in saline–alkali and normal soils. ns indicates non-significance; * indicates significant differences at p ≤ 0.05; ** indicates significant differences at p ≤ 0.01; data were expressed as mean ± SD (n = 3).

Figure 7

Influence of AMF on nutrient ions in peanut rhizosphere at mature stage in saline–alkali and normal soils. ns indicates non-significance; * indicates significant differences at p ≤ 0.05; ** indicates significant differences at p ≤ 0.01; data were expressed as mean ± SD (n = 3).