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. 1986 Jun;39(3):105-18.
doi: 10.7883/yoken1952.39.105.

Proposal of standardized methods and reference for assaying recombinant human tumor necrosis factor

S Yamazaki, E Onishi, K Enami, K Natori, M Kohase, H Sakamoto, M Tanouchi, H Hayashi

Proposal of standardized methods and reference for assaying recombinant human tumor necrosis factor

S Yamazaki et al. Jpn J Med Sci Biol. 1986 Jun.

Abstract

Two assay methods for recombinant human tumor necrosis factor (rH-TNF) were developed, one a biological L-cell assay and the other an enzyme-linked immunosorbent assay. The accuracy and reproducibility of each and the correlation between the two were studied. As a result of this investigation, the two assay methods were found appropriate for standardization of rH-TNF. A freeze-dried reference was prepared, and examination of its potency and stability showed it to be suitable for use as a reference standard for rH-TNF assays.

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