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. 1979 Jan;3(1):45-50.
doi: 10.1016/0309-1651(79)90067-5.

Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts

A D Bershadsky, V I Gelfand, T M Svitkina, I S Tint

Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts

A D Bershadsky et al. Cell Biol Int Rep. 1979 Jan.

Abstract

Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 degrees C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 degrees C.

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