Size of poly (A) +-mRNA in Saccharomyces cerevisiae polysomes
- PMID: 378427
- DOI: 10.1016/0309-1651(79)90117-6
Size of poly (A) +-mRNA in Saccharomyces cerevisiae polysomes
Abstract
The size of poly (A) +-mRNA in different classes of yeast polysomes is estimated. The average molecular weight of long-term labelled polysomal poly (A) +-mRNA is about 0,65 x 10(6) daltons. Approximately 60% of the poly (A) +-mRNA polynucleotide chains located at the 5' end, are unprotected by ribosomes and degraded by nucleases upon incubation of cell lysates, to yield a population of poly (A) +-mRNA with an average molecular weight of 0,25 x 10(6) daltons.