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. 2024 Mar;138(2):361-373.
doi: 10.1007/s00414-023-03106-x. Epub 2023 Oct 16.

Internal validation of the GA118-24B Genetic Analyzer, a stable capillary electrophoresis system for forensic DNA identification

Weifeng Qu  1 , Feng Wang  2 , Dan Sun  2 , Yi Liu  1 , Xin Jin  3 , Zheng Gong  4 , Jinjie Liu  5 , Ningjie Zhang  2 , Dan Wen  1 , Chudong Wang  1 , Hongtao Jia  1 , Ruyi Xu  1 , Xuan Tang  1 , Siqi Chen  1 , Xiaoyi Fu  1 , Xue Li  1 , Haibo Rong  2 , Tao Zhang  2 , Chuan Jin  2 , Li Chen  2 , Jienan Li  1 , Ying Liu  1 , Jifeng Cai  1 , Bowei Jiang #  6 , Lagabaiyila Zha #  7
Affiliations

Internal validation of the GA118-24B Genetic Analyzer, a stable capillary electrophoresis system for forensic DNA identification

Weifeng Qu et al. Int J Legal Med. 2024 Mar.

Abstract

The GA118-24B Genetic Analyzer (hereafter, "GA118-24B") is an independently developed capillary electrophoresis instrument. In the present research, we designed a series of validation experiments to test its performance at detecting DNA fragments compared to the Applied Biosystems 3500 Genetic Analyzer (hereafter, "3500"). Three commercially available autosomal short tandem repeat multiplex kits were used in this validation. The results showed that GA118-24B had acceptable spectral calibration for three kits. The results of accuracy and concordance studies were also satisfactory. GA118-24B showed excellent precision, with a standard deviation of less than 0.1 bp. Sensitivity and mixture studies indicated that GA118-24B could detect low-template DNA and complex mixtures as well as the results generated by 3500 in parallel experiments. Based on the experimental results, we set specific analytical and stochastic thresholds. Besides, GA118-24B showed superiority than 3500 within certain size ranges in the resolution study. Instead of conventional commercial multiplex kits, GA118-24B performed stably on a self-developed eight-dye multiplex system, which were not performed on 3500 Genetic Analyzer. We compared our validation results with those of previous research and found our results to be convincing. Overall, we conclude that GA118-24B is a stable and reliable genetic analyzer for forensic DNA identification.

Keywords: GA118-24B Genetic Analyzer; Internal validation; Polymerase chain reaction; Short tandem repeat.

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