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. 2023 Dec;28(6):442-448.
doi: 10.1016/j.slast.2023.10.002. Epub 2023 Oct 14.

Point-of-care biomarker assay for rapid multiplexed detection of CRP and IP-10

Claire S Wilson  1 , Bhavya Vashi  1 , Pavol Genzor  1 , Melissa K Gregory  1 , Jason Yau  2 , Lauren Wolfe  2 , Michael J Lochhead  2 , Phil Papst  2 , Kristen Pettrone  1 , Paul W Blair  1 , Subramaniam Krishnan  1 , Josh G Chenoweth  3 , Danielle V Clark  1
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Free article

Point-of-care biomarker assay for rapid multiplexed detection of CRP and IP-10

Claire S Wilson et al. SLAS Technol. 2023 Dec.
Free article

Abstract

Rapid and accurate measurements of immune protein markers are essential for diagnosis and treatment in all clinical settings. The recent pandemic has revealed a stark need for developing new tools and assays that could be rapidly used in diverse settings and provide useful information to clinicians. Here, we describe the development and test application of a novel one-step CRP/IP-10 duplex assay for the LightDeck platform capable of delivering reproducible and accurate measurements in under eight minutes. We used the optimized assay to measure CRP and IP-10 levels in human blood and serum samples from healthy, SARS-CoV-2 (COVID-19) positive, and influenza-like illness (ILI) presenting patients. Our results agreed with previously published analyte levels and enabled us to make statistically significant comparisons relevant to multiple clinical parameters. Our duplex assay is a simple and powerful tool for aiding prognostic decision-making in diverse settings.

Keywords: COVID-19; CRP; CXCL10; IP-10; LightDeck.

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Conflict of interest statement

Declaration of Conflicting Interests The authors declare the following competing financial interests: LightDeck Diagnostics, Inc. is a commercial organization. J.Y., L.W., M.J.L, and P.P. are/were employees of LightDeck Diagnostics.

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