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. 2023 Oct;28(4):250-257.
doi: 10.17712/nsj.2023.4.20230028.

The role of MicroRNA as a potential diagnostic and prognostic biomarker for cerebral aneurysms

Hosam M Al-Jehani  1 , Alaa N Turkistani  1 , Majd M Alrayes  1 , May A AlHamid  1 , Arwa S AlShamikh  1 , Zhra M Al Sadah  1 , Faisal M Alabbas  1 , Cyril Cyrus  1 , Amein K Al-Ali  1
Affiliations

The role of MicroRNA as a potential diagnostic and prognostic biomarker for cerebral aneurysms

Hosam M Al-Jehani et al. Neurosciences (Riyadh). 2023 Oct.

Abstract

Objectives: To identify the dysregulated functional miRNAs, miRNA-16, miRNA-143, and miRNA-200 as potential biomarkers of cerebral aneurysms(CAs) to aid in diagnosis and prognosis.

Methods: This is a prospective case-control study conducted among patients with CA. All patients' computed tomography angiography (CTA) and interventional angiogram were assessed and studied. The miRNAs were isolated and quantified from peripheral blood samples and the expression profiling was done using TaqMan chemistry on Real-Time PCR.

Results: A total of 37 samples were included. Three cases had double aneurysms and 10 cases presented with aneurysmal rupture. miRNA-16, miRNA-143, and miRNA-200 were upregulated with an absolute fold-change of >8 in the CA group in comparison to controls (p<0.05). miRNA-200 showed double expression in patients with single aneurysm. A statically significant increase was observed in the expression of miRNA-143 in patients who had an aneurysmal rupture with p<0.05. Diabetic patients showed an obvious increase in miRNA-200 (13.03 folds) and miRNA-16 (26.82 folds) expression. Also, there was a notable elevation in miRNA-16, miRNA-143, and miRNA-200 in patients who had hypertension in comparison to those who did not.

Conclusion: miRNA-16, miRNA-143, and miRNA-200 showed statically significant higher expression among cases with cerebral aneurysms in comparison to controls. Thus, these preliminary results of miRNAs biomarkers are promising future tool to be used for aneurysmal screening.

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Figures

Figure 1
Figure 1
- This graph shows the miRNAs expressional fold changes between healthy controls and CA cases, the mean expression of the controls was set as 1 in which we considered it to be the reference point to measure the expressional fold changes of the cases in regard to this reference point.
Figure 2
Figure 2
- MiRNA expression of CA cases based on gender, showing almost the doubling of miRNA-143 in males in comparison to females.
Figure 3
Figure 3
- miRNA expression based on the multiplicity of aneurysms, showing higher expression among cases who had a single aneurysm in comparison to those who had two aneurysms.
Figure 4
Figure 4
- miRNA expression based on the presence aneurysmal rupture, miRNA-143 was highly expressed among cases who had aneurysmal rupture.
See this image and copyright information in PMC

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