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. 2023 Dec;65(12):2587-2603.
doi: 10.1111/jipb.13574. Epub 2023 Nov 17.

Paternally imprinted LATE-FLOWERING2 transcription factor contributes to paternal-excess interploidy hybridization barriers in wheat

Guanghui Yang  1 , Man Feng  1 , Kuohai Yu  1 , Guangxian Cui  1 , Yan Zhou  1 , Lv Sun  1 , Lulu Gao  1 , Yumei Zhang  2 , Huiru Peng  1 , Yingyin Yao  1 , Zhaorong Hu  1 , Vincenzo Rossi  3 , Ive De Smet  4   5 , Zhongfu Ni  1 , Qixin Sun  1 , Mingming Xin  1
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Paternally imprinted LATE-FLOWERING2 transcription factor contributes to paternal-excess interploidy hybridization barriers in wheat

Guanghui Yang et al. J Integr Plant Biol. 2023 Dec.

Abstract

Interploidy hybridization between hexaploid and tetraploid genotypes occurred repeatedly during genomic introgression events throughout wheat evolution, and is commonly employed in wheat breeding programs. Hexaploid wheat usually serves as maternal parent because the reciprocal cross generates progeny with severe defects and poor seed germination, but the underlying mechanism is poorly understood. Here, we performed detailed analysis of phenotypic variation in endosperm between two interploidy reciprocal crosses arising from tetraploid (Triticum durum, AABB) and hexaploid wheat (Triticum aestivum, AABBDD). In the paternal- versus the maternal-excess cross, the timing of endosperm cellularization was delayed and starch granule accumulation in the endosperm was repressed, causing reduced germination percentage. The expression profiles of genes involved in nutrient metabolism differed strongly between these endosperm types. Furthermore, expression patterns of parental alleles were dramatically disturbed in interploidy versus intraploidy crosses, leading to increased number of imprinted genes. The endosperm-specific TaLFL2 showed a paternally imprinted expression pattern in interploidy crosses partially due to allele-specific DNA methylation. Paternal TaLFL2 binds to and represses a nutrient accumulation regulator TaNAC019, leading to reduced storage protein and starch accumulation during endosperm development in paternal-excess cross, as confirmed by interploidy crosses between tetraploid wild-type and clustered regularly interspaced palindromic repeats (CRISPR) - CRISPR-associated protein 9 generated hexaploid mutants. These findings reveal a contribution of genomic imprinting to paternal-excess interploidy hybridization barriers during wheat evolution history and explains why experienced breeders preferentially exploit maternal-excess interploidy crosses in wheat breeding programs.

Keywords: imprinting; interploidy cross; wheat.

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References

REFERENCES

    1. Appels, R., Eversole, K., Stein, N., Feuillet, C., Keller, B., Rogers, J., Pozniak, C.J., Choulet, F., Distelfeld, A., Poland, J., et al. (2018). Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science 361: eaar7191.
    1. Batista, R.A., and Köhler, C. (2020). Genomic imprinting in plants-revisiting existing models. Genes Dev. 34: 24-36.
    1. Batista, R.A., Moreno-Romero, J., Qiu, Y., van Boven, J., Santos-González, J., Figueiredo, D.D., and Köhler, C. (2019). The MADS-box transcription factor PHERES1 controls imprinting in the endosperm by binding to domesticated transposons. eLife 8: e50541.
    1. Bolger, A.M., Lohse, M., and Usadel, B. (2014). Trimmomatic: A flexible trimmer for Illumina sequence data. Bioinformatics 30: 2114-2120.
    1. Burkart-Waco, D., Ngo, K., Lieberman, M., and Comai, L. (2015). Perturbation of parentally biased gene expression during interspecific hybridization. PLoS ONE 10: e0117293.

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