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. 2023 Oct 17;46(10):100.
doi: 10.1140/epje/s10189-023-00360-0.

Pincus blob elasticity in an intrinsically disordered protein

Hoang P Truong  1 , Ian L Morgan  2 , Gil Koren  3   4 , Ram Avinery  3   4 , Roy Beck  3   4 , Omar A Saleh  5   6   7
Affiliations

Pincus blob elasticity in an intrinsically disordered protein

Hoang P Truong et al. Eur Phys J E Soft Matter. .

Erratum in

Abstract

Understanding the dynamic structure of intrinsically disordered proteins (IDPs) is important to deciphering their biological functions. Here, we exploit precision entropic elasticity measurements to infer the conformational behavior of a model IDP construct formed from the disordered tail of the neurofilament low molecular weight protein. The IDP construct notably displays a low-force power-law elastic regime, consistent with the Pincus blob model, which allows direct extraction of the Flory exponent, [Formula: see text], from the force-extension relationship. We find [Formula: see text] increases with added denaturant, transitioning from a nearly ideal chain to a swollen chain in a manner quantitatively consistent with measurements of IDP dimensions from other experimental techniques. We suggest that measurements of entropic elasticity could be broadly useful in the study of IDP structure.

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