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. 1986 Aug;6(8):2903-9.
doi: 10.1128/mcb.6.8.2903-2909.1986.

Genetic analysis of the human thymidine kinase gene promoter

J A Kreidberg, T J Kelly

Genetic analysis of the human thymidine kinase gene promoter

J A Kreidberg et al. Mol Cell Biol. 1986 Aug.

Abstract

The promoter of the human thymidine kinase gene was defined by DNA sequence and genetic analyses. Mutant plasmids with deletions extending into the promoter region from both the 5' and 3' directions were constructed. The mutants were tested in a gene transfer system for the ability to transform TK- cells to the TK+ phenotype. This analysis delimited the functional promoter to within an 83-base-pair region upstream of the mRNA cap site. This region contains sequences common to other eucaryotic promoters including G X C-rich hexanucleotides, a CAAT box, and an A X T-rich region. The CAAT box is in an inverted orientation and is part of a 9-base-pair sequence repeated twice in the promoter region. Comparison of the genomic sequence with the cDNA sequence defined the first exon of the thymidine kinase gene.

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