Two plant-growth-promoting Bacillus species can utilize nanoplastics

Abstract

Plastics in agricultural soils pose a potential risk to humans because environmental plastics can enter our foods. Here, we present a first step toward developing bacteria that can both flourish in agricultural settings and bioremediate nanoplastics. We exposed two species known to promote plant growth in agricultural settings, Bacillus inaquosorum and B. velezensis, to polystyrene nanoplastic beads at various dosages. When grown in a medium with a low dosage of plastic as the only carbon source, the bacteria could oxidize the plastic, indicating the possibility of utilizing the plastic in their growth. When plastic was added to a rich medium, low and high dosages brought immediate death or inhibition to about a third of B. inaquosorum cells during 1 h. Despite the immediate harm, over the course of 24 h, the bacteria from one strain each of B. inaquosorum and B. velezensis reached higher densities at low plastic doses than with no plastic, although they reached lower densities at high plastic doses (a toxicological phenomenon known as hormesis). Microscopic studies demonstrated that the bacteria are shielded from excessive accumulation of nanoplastic particles. Because these plant-growth-promoting species can utilize polystyrene nanoplastics, strains of these species might be developed to bioremediate environmental plastic in agricultural settings. SYNOPSIS: Plastic fragments on farmlands accumulate on produce, creating a need for bioremediation. We identify bacteria that can flourish on agricultural land and utilize nanoplastics, a first step toward developing agricultural bioremediators.

Keywords: Bacillus; Hormetic response; Nanoplastics; Plastic pollution; Polystyrene particles.