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. 2023 Oct 21;13(1):17993.
doi: 10.1038/s41598-023-45341-7.

Assessment of expression of calcium signaling related lncRNAs in epilepsy

Mohammad Taheri  1   2 , Ashkan Pourtavakoli  3 , Solat Eslami  4   5 , Soudeh Ghafouri-Fard  6 , Arezou Sayad  7
Affiliations

Assessment of expression of calcium signaling related lncRNAs in epilepsy

Mohammad Taheri et al. Sci Rep. .

Abstract

Calcium signaling is a metabolic pathway that is essential in neurons development and can be involved in the pathobiology of epilepsy. We assessed expression of three mRNA coding gene (SLC1A1, SLC25A12, and ATP2B2) and three related long non-coding RNAs (LINC01231:1, lnc-SLC25A12-8:1 and lnc-MTR-1:1) from this pathway in 39 patients with refractory epilepsy and 71 healthy controls. Expression of all genes except for lnc-SLC25A12 was higher in total epileptic cases compared with controls (P values = 0.0002, < 0.0001, < 0.0001, 0.049 and 0.0005 for SLC1A1, SLC25A12, LINC01231, ATP2B2 and lnc-MTR-1, respectively. When we separately compared expression of genes among males and females, SLC1A1, SLC25A12, LINC01231 and lnc-MTR-1 showed up-regulation in male cases compared with male controls. Moreover, expressions of SLC1A1 and SLC25A12 were higher in female cases compared with female controls. Remarkably, SLC25A12 was found to have the highest sensitivity value (= 1) for differentiation of epileptic cases from controls. Moreover, lnc-MTR-1 and lnc-SLC25A12 were sensitive markers for such purpose (sensitivity values = 0.89 and 0.87, respectively). The highest value belonged to LINC01231 with the value of 0.76. Taken together, this study demonstrates dysregulation of calcium-signaling related genes in epileptic patients and suggests these genes as potential biomarkers for epilepsy.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Expression of three genes encoding ion channels and transporters including SLC1A1, SLC25A12, ATP2B2 and their related long non-coding RNAs, namely LINC01231, lnc-SLC25A12, and lnc-MTR-1 in epilepsy patients and healthy controls as described by − delta Ct values. Following the normality test, unpaired t test (for SLC25A12 and lnc-SLC25A12) and non-parametric test (Mann–Whitney U test) (for SLC1A1, ATP2B2, LINC01231 and lnc-MTR-1) was used to identify differentially expressed genes between two groups (* < 0.05, ***P value < 0.001 and ****P value < 0.0001).
Figure 2
Figure 2
ROC curves of six studied genes in patients with epilepsy disease.
See this image and copyright information in PMC

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