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Review
. 2023 Dec;66(14):444-451.
doi: 10.1002/jlcr.4069. Epub 2023 Oct 24.

Site-specifically radiolabeled nanobodies for imaging blood-brain barrier penetration and targeting in the brain

Yingbo Li  1 , Junfeng Wang  2
Affiliations
Review

Site-specifically radiolabeled nanobodies for imaging blood-brain barrier penetration and targeting in the brain

Yingbo Li et al. J Labelled Comp Radiopharm. 2023 Dec.

Abstract

Nanobodies (Nbs) hold significant potential in molecular imaging due to their unique characteristics. However, there are challenges to overcome when it comes to brain imaging. To address these obstacles, collaborative efforts and interdisciplinary research are needed. This article aims to raise awareness and encourage collaboration among researchers from various fields to find solutions for effective brain imaging using Nbs. By fostering cooperation and knowledge sharing, we can make progress in overcoming the existing limitations and pave the way for improved molecular imaging techniques in the future.

Keywords: BBB penetration; brain disorder; molecular imaging; nanobody; site-specific labeling.

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Conflict of interest statement

Conflict of Interest

There are no conflicts of interest to declare.

Figures

Figure 1.
Figure 1.
The various antibody formats: conventional antibody (mAb), camel heavy-chain antibody and nanobody (VHH or Nb).(13) And an illustration of CDRs from a recent publication about AT1R nanobody AT118.(14)
Figure 2.
Figure 2.
Ex vivo autoradiography on sagittal sections prepared from wild type mouse brains isolated at 8 h post administration (From reference 56).
See this image and copyright information in PMC

References

    1. Ghosh KK, Padmanabhan P, Yang C-T, Ng DCE, Palanivel M, Mishra S, Halldin C, Gulyás B. Positron emission tomographic imaging in drug discovery. Drug Discovery Today. 2022;27(1):280–91. doi: 10.1016/j.drudis.2021.07.025. - DOI - PubMed
    1. Abraham T, Feng J. Evolution of brain imaging instrumentation. Seminars in nuclear medicine. 2011;41(3)202–19. doi:10.1053/j.semnuclmed.2010.12.001. - DOI - PubMed
    1. Pike VW. PET radiotracers: crossing the blood–brain barrier and surviving metabolism. Trends in pharmacological sciences. 2009;30(8):431–40. doi:10.1016/j.tips.2009.05.005 - DOI - PMC - PubMed
    1. Holland JP, Liang SH, Rotstein BH, Collier TL, Stephenson NA, Greguric I, Vasdev N. Alternative approaches for PET radiotracer development in Alzheimer’s disease: imaging beyond plaque. Journal of Labelled Compounds and Radiopharmaceuticals. 2014;57(4):323–31. doi:10.1002/jlcr.3158. - DOI - PubMed
    1. W Pike V Considerations in the development of reversibly binding PET radioligands for brain imaging. Current Medicinal Chemistry. 2016;23(18):1818–69. doi:10.2174/0929867323666160418114826. - DOI - PMC - PubMed

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