Coordination of hydraulic and leaf-level gas exchange traits during water-deficit acclimation in apple rootstocks

Abstract

Water deficit episodes impact apple (Malus domestica) productivity through challenging the trees' water status, the influence of extreme high temperature climate has become increasingly prominent in recent years. Rootstocks can bestow specific properties on the fruit trees such as the resistance to drought stress. However, the related hydraulic mechanisms in response to water deficit have not been fully understood. Herein, five rootstocks (SH6, GM256, M9, M26, and MM106) were examined under water limitation. The hydraulic conductance of root (Kroot), shoots (Kshoot), and stems (Kstem-shoot) in the five rootstocks reduced slightly during drought stress. Whereas the leaf water potential and photosynthesis of five rootstocks decreased dramatically when they were exposed to drought stress. Additionally, the Kshoot and Kstem-shoot were strongly correlated with the total plant leaf area. Aquaporins (AQPs) involved in the symplastic water transport pathway, the PIP2:1, TIP1:1, and TIP2:2 mRNA levels of all genotypic rootstocks showed significant regulation under drought stress. We examined the relationships among photosynthesis, apoplastic, and symplastic water movement pathways to achieve a comprehensive understanding of rootstocks' hydraulic strategy for improving drought adaptation. The PIP2:1 and TIP2:1 in leaves were more sensitive to root hydraulic conductance in response to drought stress. Furthermore, the coordinated relationship existed in leaf-specific conductance of shoot (K_l -shoot) and transpiration rate (Tr) under drought stress in the rootstocks. Overall, the drought resistance in the five dwarfing rootstocks is associated with the rapid re-establishment of water-related traits, and the effect of the canopy on the drought resistance in apple rootstocks merits much more attention.