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. 2023 Dec;46(12):1791-1799.
doi: 10.1007/s00449-023-02935-9. Epub 2023 Oct 26.

Kinetic and stoichiometric parameters in the fed-batch bioreactor production of poly(3-hydroxybutyrate) by Bacillus megaterium using different carbon sources

Rafaela Nery De Melo  1 , Guilherme de Souza Hassemer do  1 , Lucas Henrique Nascimento  1 , Rosicler Colet  1 , Clarice Steffens  2 , Alexander Junges  1 , Eunice Valduga  1
Affiliations

Kinetic and stoichiometric parameters in the fed-batch bioreactor production of poly(3-hydroxybutyrate) by Bacillus megaterium using different carbon sources

Rafaela Nery De Melo et al. Bioprocess Biosyst Eng. 2023 Dec.

Abstract

This study investigates the effects of different strategies on poly(3-hydroxybutyrate)-P(3HB) production in a fed-batch bioreactor by Bacillus megaterium using candy industry effluent (CIE), sucrose, and rice parboiled water (RPW) as carbon sources. In biosynthesis, kinetic and stoichiometric parameters of substrate conversion into products and/or cells, productivity, instantaneous, and specific conversion rates were evaluated. The maximum concentration of P(3HB) was 4.00 g.L-1 (77% of the total dry mass) in 42 h of cultivation in minimal medium/RPW added with a carbon source based on CIE, demonstrating that the fed-batch provided an increase of approximately 22% in the polymer concentration and 32% in the overall productivity in relation to medium based on commercial sucrose. Fed-batch cultivation also had the advantage of avoiding the extra time required for inoculum preparation and sterilization of the bioreactor during the batch, which thereby increased the overall industrial importance of the process. Effluents from the candy, confectionery, and/or rice parboiling industries can be used as alternative substrates for P(3HB) production at a low cost.

Keywords: Biopolymer; Candy industry effluent; Kinetic parameters; Polyhydroxyalkanoates; Rice parboiling water.

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References

    1. Obruca S, Sedlacek P, Slaninova E et al (2020) Novel unexpected functions of PHA granules. Appl Microbiol Biotechnol 104:4795–4810. https://doi.org/10.1007/s00253-020-10568-1 - DOI - PubMed
    1. Hassemer G, Colet R, Melo RN et al (2021) Production of Poly(3-hydroxybutyrate) (P(3HB)) from different agroindustry byproducts by Bacillus megaterium. Biointerface Res Appl Chem 11:14278–14289. https://doi.org/10.33263/BRIAC116.1427814289 - DOI
    1. Yeo JCC, Muiruri JK, Thitsartarn W et al (2018) Recent advances in the development of biodegradable PHB-based toughening materials: approaches, advantages and applications. Mater Sci Eng C 92:1092–1116. https://doi.org/10.1016/j.msec.2017.11.006 - DOI
    1. Mohapatra S, Maity S, Dash HR et al (2017) Bacillus and biopolymer: prospects and challenges. Biochem Biophys Rep 12:206–213. https://doi.org/10.1016/j.bbrep.2017.10.001 - DOI - PubMed - PMC
    1. Park SJ, Choi J-I, Lee SP (2005) Short-chain-length polyhydroxyalkanoates: synthesis in metabolically engineered Escherichia coli and medical applications. J Microbiol Biotechnol 15:206–215

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