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. 2023 Nov;33(5-6):449-456.
doi: 10.1007/s00572-023-01130-8. Epub 2023 Oct 26.

A cryptic root isolate belonging to Geoglossales from potted Rhododendron: its molecular phylogeny and ability to colonize an ericoid mycorrhizal host in vitro

Takashi Baba  1 , Dai Hirose  2
Affiliations

A cryptic root isolate belonging to Geoglossales from potted Rhododendron: its molecular phylogeny and ability to colonize an ericoid mycorrhizal host in vitro

Takashi Baba et al. Mycorrhiza. 2023 Nov.

Abstract

Although the lifestyle of Geoglossales remains largely unknown, recent advancements have established a hypothesis regarding the ericoid mycorrhizal lifestyle of geoglossoid fungi. In this study, we focused on one isolate of Geoglossales sp. obtained from surface-sterilized roots of potted Rhododendron transiens. We aimed to reveal the phylogenetic position and in vitro colonizing ability of this species in the hair roots of ericoid mycorrhizal plants. Based on our multigene phylogenetic tree, this species is a sister of the genus Sarcoleotia which has not been reported from either other studies or field environment. Its ascocarps could not be obtained, and conspecific sequences were not found in the databases and repositories examined. The Geoglossales sp. colonized the vital rhizodermal cells of blueberries in vitro with hyphal coils. There were relatively large morphological variations of coils consistent with extraradical hyphae; however, overall, the colonization morphologically resembled those by Sarcoleotia globosa and representative ericoid mycorrhizal fungi. The taxonomy and ecological significance of the species remain to be resolved; nevertheless, our results suggest that the ericoid mycorrhizal lifestyle may be widespread within Geoglossales.

Keywords: Earth tongues; Ericaceae; Geoglossales; Hyphal coil; In vitro resynthesis; Vital staining.

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References

    1. Arauzo S, Iglesias P (2014) La familia Geoglossaceae ss. str. en la península Ibérica la Macaronesia. Errotari 11:166–259
    1. Baba T, Hirose D (2021) Slow-growing fungi belonging to the unnamed lineage in Chaetothyriomycetidae form hyphal coils in vital ericaceous rhizodermal cells in vitro. Fungal Biol 125:1026–1035. https://doi.org/10.1016/j.funbio.2021.07.003 - DOI - PubMed
    1. Baba T, Janošík L, Koukol O, Hirose D (2021) Genetic variations and in vitro root-colonizing ability for an ericaceous host in Sarcoleotia globosa (Geoglossomycetes). Fungal Biol 125:971–979. https://doi.org/10.1016/j.funbio.2021.08.005 - DOI - PubMed
    1. Boeraeve M, Kohout P, Ceulemans T et al (2022) Changes in the root microbiome of four plant species with different mycorrhizal types across a nitrogen deposition gradient in ombrotrophic bogs. Soil Biol Biochem 169:108673. https://doi.org/10.1016/j.soilbio.2022.108673 - DOI
    1. Daghino S, Martino E, Voyron S, Perotto S (2022) Metabarcoding of fungal assemblages in Vaccinium myrtillus endosphere suggests colonization of above-ground organs by some ericoid mycorrhizal and DSE fungi. Sci Rep 12:11013. https://doi.org/10.1038/s41598-022-15154-1 - DOI - PubMed - PMC

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