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Comment
. 2023 Oct 23;3(10):100612.
doi: 10.1016/j.crmeth.2023.100612.

Immunophenotyping beyond the limits of time

J Fréderique de Graaf  1 , Ramon Arens  2
Affiliations
Comment

Immunophenotyping beyond the limits of time

J Fréderique de Graaf et al. Cell Rep Methods. .

Abstract

Immunophenotyping is a powerful approach for deciphering responses of the immune system to drug screening and immunotherapy. In this issue of Cell Report Methods, Liechti et al. have advanced this approach by developing a pipeline, which allows high-throughput but still accurate single-cell immunophenotyping in time.

Keywords: CP: immunology.

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Conflict of interest statement

Declaration of interests The authors declare no competing interests.

Figures

Figure 1
Figure 1
A pipeline for immunophenotyping in large cohorts or time-connected studies using high-dimensional flow cytometry and unsupervised data analysis Schematic representation of sample processing for precise, accurate, and consistent data acquisition using either conventional or spectral(-enabled) flow cytometry followed by unsupervised data analysis. The pipeline consists of steps that optimize sample preparation protocols, takes into consideration technical variations, validates instrumental performance, and removes low-quality events and inter-assay differences ultimately performing unsupervised data analysis via uniform manifold approximation and projection (UMAP) or FlowSOM. Figure created using BioRender (https://biorender.com).
See this image and copyright information in PMC

Comment on

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