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. 2024 Feb;20(2):132-133.
doi: 10.1038/s41589-023-01435-x.

Reducing mitochondrial mysteries

Rachel M Guerra  1   2   3 , David J Pagliarini  4   5   6
Affiliations

Reducing mitochondrial mysteries

Rachel M Guerra et al. Nat Chem Biol. 2024 Feb.

Abstract

Defining subcellular locations and interacting partners for proteins accelerates their functional characterization. A new in vivo tagging approach achieves both for mitochondrial matrix proteins and helps connect a key oxidoreductase to coenzyme Q biosynthesis.

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Figures

Figure 1.
Figure 1.. Mitochondrial matrix-specific proximity labeling iden fies RTN4IP1 as a novel oxidoreductase involved in CoQ biosynthesis.
A) The MAX-Tg mouse model involves localiza on of the APEX2 tag to the mitochondrial matrix via fusion to the mitochondrial targe ng sequence of COX4I1. Subsequent proximity labeling, bio n enrichment, and mass spectrometry is performed to characterize the matrix proteome across different muscle ssue types. B) The current model for RTN4IP1 func on proposes that it enables CoQ biosynthesis via the reduc on of COQ3’s DMeQ substrate.
See this image and copyright information in PMC

References

    1. Park I, Kim K, Kim J, Kim A-K, Bae S, Jung M, Choi J, Mishra PK, Kim T-M, Kwak C, et al. (2023). In vivo mitochondrial matrix proteome profiling reveals RTN4IP1/OPA10 as an NAD(P)H oxidoreductase for Coenzyme Q biosynthesis. Nature Chemical Biology. - PMC - PubMed
    1. Qin W, Cho KF, Cavanagh PE, and Ting AY (2021). Deciphering molecular interactions by proximity labeling. Nat. Methods 18, 133–143. 10.1038/s41592-020-01010-5. - DOI - PMC - PubMed
    1. Rath S, Sharma R, Gupta R, Ast T, Chan C, Durham TJ, Goodman RP, Grabarek Z, Haas ME, Hung WHW, et al. (2020). MitoCarta3.0: an updated mitochondrial proteome now with sub-organelle localization and pathway annotations. Nucleic Acids Res. 49, gkaa1011-. 10.1093/nar/gkaa1011. - DOI - PMC - PubMed
    1. Morgenstern M, Peikert CD, Lubbert P, Suppanz I, Klemm C, Alka O, Steiert C, Naumenko N, Schendzielorz A, Melchionda L, et al. (2021). Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context. Cell Metab 33, 2464–2483 e18. 10.1016/j.cmet.2021.11.001. - DOI - PMC - PubMed
    1. Sung AY, Floyd BJ, and Pagliarini DJ (2020). Systems Biochemistry Approaches to Defining Mitochondrial Protein Function. Cell Metabolism 31, 669–678. 10.1016/j.cmet.2020.03.011. - DOI - PMC - PubMed

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