Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jan;68(1):e2300491.
doi: 10.1002/mnfr.202300491. Epub 2023 Oct 27.

Dietary Amino Acid Source Elicits Sex-Specific Metabolic Response to Diet-Induced NAFLD in Mice

Clémence Rives  1 , Céline Marie Pauline Martin  1 , Lauris Evariste  1 , Arnaud Polizzi  1 , Marine Huillet  1 , Frédéric Lasserre  1 , Valérie Alquier-Bacquie  1 , Prunelle Perrier  1 , Jelskey Gomez  1 , Yannick Lippi  1 , Claire Naylies  1 , Thierry Levade  2   3 , Frédérique Sabourdy  2   3 , Hervé Remignon  1   4 , Pierre Fafournoux  5 , Benoit Chassaing  6 , Nicolas Loiseau  1 , Hervé Guillou  1 , Sandrine Ellero-Simatos  1 , Laurence Gamet-Payrastre  1 , Anne Fougerat  1
Affiliations

Dietary Amino Acid Source Elicits Sex-Specific Metabolic Response to Diet-Induced NAFLD in Mice

Clémence Rives et al. Mol Nutr Food Res. 2024 Jan.

Abstract

Scope: Non-alcoholic fatty liver disease (NAFLD) is a sexually dimorphic disease influenced by dietary factors. Here, the metabolic and hepatic effects of dietary amino acid (AA) source is assessed in Western diet (WD)-induced NAFLD in male and female mice.

Methods and results: The AA source is either casein or a free AA mixture mimicking the composition of casein. As expected, males fed a casein-based WD display glucose intolerance, fasting hyperglycemia, and insulin-resistance and develop NAFLD associated with changes in hepatic gene expression and microbiota dysbiosis. In contrast, males fed the AA-based WD show no steatosis, a similar gene expression profile as males fed a control diet, and a distinct microbiota composition compared to males fed a casein-based WD. Females are protected against WD-induced liver damage, hepatic gene expression, and gut microbiota changes regardless of the AA source.

Conclusions: Free dietary AA intake prevents the unhealthy metabolic outcomes of a WD preferentially in male mice.

Keywords: NAFLD; amino acids; liver metabolism; microbiota; sex dimorphism.

PubMed Disclaimer

References

    1. Z. M. Younossi, A. B. Koenig, D. Abdelatif, Y. Fazel, L. Henry, M. Wymer, Hepatology 2016, 64, 73.
    1. A. C. Sheka, O. Adeyi, J. Thompson, B. Hameed, P. A. Crawford, S. Ikramuddin, JAMA 2020, 323, 1175.
    1. P. Lefebvre, B. Staels, Nat. Rev. Endocrinol. 2021, 17, 662.
    1. S. Smati, Gut 2022, 71, 807.
    1. J. Vandel, J. Dubois-Chevalier, C. Gheeraert, B. Derudas, V. Raverdy, D. Thuillier, L. Gaal, S. Francque, F. Pattou, B. Staels, J. Eeckhoute, P. Lefebvre, Hepatology 2021, 73, 920.