Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Nov;39(5):661-8.

High molecular weight DNA from fixed cytogenetic preparations

P E Barker, J R Testa, N Z Parsa, R Snyder

High molecular weight DNA from fixed cytogenetic preparations

P E Barker et al. Am J Hum Genet. 1986 Nov.

Abstract

Cell pellets that have been stored in routine clinical cytogenetic fixative were studied for the presence of intact DNA. A method for the isolation of high molecular weight DNA from fixed cytogenetic preparations of human leukocytes, bone marrow, and cell hybrid cultures is presented. DNA preparations from fixed pellets were cleaved with restriction enzymes, transferred to nitrocellulose filters after agarose gel electrophoresis, and hybridized to radiolabeled probes to demonstrate that fixed cell pellets could yield DNA of sufficient quality for Southern blot hybridization analysis. This protocol may be useful for molecular analysis of DNA from fixed cell pellets of patients who are unavailable for additional sampling.

PubMed Disclaimer

References

    1. Exp Cell Res. 1968 Apr;50(1):47-53 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. Nucleic Acids Res. 1976 Sep;3(9):2303-8 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5631-5 - PubMed

Publication types

LinkOut - more resources