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. 2023 Oct 13;11(10):2780.
doi: 10.3390/biomedicines11102780.

Synergistic Antimicrobial Effect of Cold Atmospheric Plasma and Redox-Active Nanoparticles

Artem M Ermakov  1   2   3 , Vera A Afanasyeva  1   2 , Alexander V Lazukin  4 , Yuri M Shlyapnikov  2 , Elizaveta S Zhdanova  1   2 , Anastasia A Kolotova  2 , Artem S Blagodatski  2 , Olga N Ermakova  2 , Nikita N Chukavin  2   5 , Vladimir K Ivanov  6 , Anton L Popov  2   5
Affiliations

Synergistic Antimicrobial Effect of Cold Atmospheric Plasma and Redox-Active Nanoparticles

Artem M Ermakov et al. Biomedicines. .

Abstract

Cold argon plasma (CAP) and metal oxide nanoparticles are well known antimicrobial agents. In the current study, on an example of Escherichia coli, a series of analyses was performed to assess the antibacterial action of the combination of these agents and to evaluate the possibility of using cerium oxide and cerium fluoride nanoparticles for a combined treatment of bacterial diseases. The joint effect of the combination of cold argon plasma and several metal oxide and fluoride nanoparticles (CeO2, CeF3, WO3) was investigated on a model of E. coli colony growth on agar plates. The mutagenic effect of different CAP and nanoparticle combinations on bacterial DNA was investigated, by means of a blue-white colony assay and RAPD-PCR. The effect on cell wall damage, using atomic force microscopy, was also studied. The results obtained demonstrate that the combination of CAP and redox-active metal oxide nanoparticles (RAMON) effectively inhibits bacterial growth, providing a synergistic antimicrobial effect exceeding that of any of the agents alone. The combination of CAP and CeF3 was shown to be the most effective mutagen against plasmid DNA, and the combination of CAP and WO3 was the most effective against bacterial genomic DNA. The analysis of direct cell wall damage by atomic force microscopy showed the combination of CAP and CeF3 to be the most effective antimicrobial agent. The combination of CAP and redox-active metal oxide or metal fluoride nanoparticles has a strong synergistic antimicrobial effect on bacterial growth, resulting in plasmid and genomic DNA damage and cell wall damage. For the first time, a strong antimicrobial and DNA-damaging effect of CeF3 nanoparticles has been demonstrated.

Keywords: antimicrobial effect; bacteria; cerium fluoride; cerium oxide; cold argon plasma; combined treatment; nanoparticles; tungsten oxide.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
E. coli growth inhibition zones after CAP treatment. Control plate (0 min)—no CAP treatment, no NPs.
Figure 2
Figure 2
Antimicrobial and mutagenic effects of CAP in combination with CeO2, CeF3 and WO3 NPs, as assessed using blue/white colouring assay on E. coli (a). Blue/white colony ratio after CAP treatment (b).
Figure 3
Figure 3
Genomic DNA template stability (GTS) of E. coli.
Figure 4
Figure 4
Representative AFM images of bacteria after treatment with CAP, nanoparticles or combinations of these. The scan area was 30–100 µm2; a typical z-axis scale is 200 nm.
See this image and copyright information in PMC

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