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. 2023 Oct 11;11(10):2535.
doi: 10.3390/microorganisms11102535.

Leucobacter manosquensis sp. nov.-A Novel Bacterial Species Isolated from Healthy Human Skin

Manon Boxberger  1   2 , Sibylle Magnien  1   2 , Angeline Antezack  1   2   3   4 , Clara Rolland  1   2 , Marine Makoa Meng  1   2 , Cheikh Ibrahima Lo  1 , Bernard La Scola  1   2 , Nadim Cassir  1   2
Affiliations

Leucobacter manosquensis sp. nov.-A Novel Bacterial Species Isolated from Healthy Human Skin

Manon Boxberger et al. Microorganisms. .

Abstract

Extending our knowledge on human skin microbiota is a challenge to better decipher its role in health and disease. Using the culturomics method, we isolated strain Marseille-Q4368 from the healthy forehead of a 59-year-old woman. We describe here the main characteristics of this bacterium using a taxonogenomic approach. This new bacterial species is Gram-positive, non-motile, and non-spore-forming. Its 16S rRNA sequence exhibited a similarity of 99.59% with Leucobacter chromiiresistens, the most closely related species in terms of nomenclature. However, a digital DNA-DNA hybridization analysis between these two species revealed a maximum identity similarity of only 27.5%. We found phenotypical and genomic differences between strain Marseille-Q4368 and its closely related species. These findings underscore the classification of this bacterium as a distinct species. Hence, we propose the name Leucobacter manosquensis sp. nov. strain Marseille-Q4368 (=CSUR Q4368 = DSM 112403) for this newly identified bacterial species.

Keywords: Leucobacter; bacteria; culturomics; human skin microbiota; new species; taxonogenomics.

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Conflict of interest statement

The authors declare no conflict of interest. M.B. has a Ph.D. grant due to the collaboration between M&L Laboratories and Aix Marseille University reference PVM:2018-200.

Figures

Figure 1
Figure 1
(A) 16S rRNA-based phylogenetic tree (B) Whole-genome-based phylogenetic tree highlighting the position of L. manosquensis sp nov., strain Marseille-Q4368T relative to other closely related bacterial taxa.
Figure 2
Figure 2
Scanning electron microscopy of L. manosquensis sp. nov., strain Marseille-Q4368T using a TM4000 microscope (Hitachi High-Tech, HHT, Tokyo, Japan). The scale bar represents 10 µm.
Figure 3
Figure 3
Graphical circular map of the genome from L. manosquensis strain Marseille-Q4368T obtained using the CGView Server online tool [30].
See this image and copyright information in PMC

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