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. 1979 Aug;139(2):597-607.
doi: 10.1128/jb.139.2.597-607.1979.

Isolation and characterization of replication-deficient mutants of ColE1 plasmids

T Hashimoto-Gotoh, J Inselburg

Isolation and characterization of replication-deficient mutants of ColE1 plasmids

T Hashimoto-Gotoh et al. J Bacteriol. 1979 Aug.

Abstract

Replication-defective mutants of plasmid ColE1 were isolated from a chimeric plasmid formed by ligating a temperature-sensitive replication derivative of pSC101, pHSG1, with a ColE1-Tn3-containing plasmid. The replication-defective ColE1 mutants isolated were all spontaneous deletion mutants that had lost the ColE1 replication origin and regions adjacent to it. The extent of a deletion was determined by analyzing restriction endonuclease-generated deoxyribonucleic acid fragments of the ColE1 plasmid component of the chimeras by both agarose and polyacrylamide gel electrophoresis. None of the chimeras containing the replication-defective ColE1 mutants was able to replicate in the presence of chloramphenicol. The expression of ColE1 incompatibility was either markedly reduced or not detectable in the replication mutants isolated.

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References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5265-9 - PubMed
    1. J Bacteriol. 1977 Jan;129(1):482-91 - PubMed
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