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. 2023 Nov;13(11):e1456.
doi: 10.1002/ctm2.1456.

Unveiling the neuroinflammatory pathogenesis of persistent functional dyspepsia in H. pylori infection: Insights on MMP-9 as a therapeutic target

Yujen Tseng  1 , Lingxi Lin  1 , Shaocong Mo  1 , Suhan Zhao  1 , Qiwei Shen  2 , Huan Song  1 , Haoshu Cui  1 , Jun Zhang  1 , Wanwei Zheng  1 , Zhongguang Luo  1 , Feifei Luo  1 , Jie Liu  1
Affiliations

Unveiling the neuroinflammatory pathogenesis of persistent functional dyspepsia in H. pylori infection: Insights on MMP-9 as a therapeutic target

Yujen Tseng et al. Clin Transl Med. 2023 Nov.
No abstract available

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Figures

FIGURE 1
FIGURE 1
Disruption of gastric ENS in H. pylori infection. (A) Tissue clearing of gastric mucosal biopsy showed a disruption in the neural structure in H. pylori infection compared to normal control; (B) Full‐thickness immunofluorescent staining showed a decrease in PGP9.5 staining in H. pylori infection in the muscularis mucosae layer; (C) A significant decrease in neuron density and neuroinflammatory score was noted in H. pylori infection; (D) external validation confirmed a significant decrease in neuron density and neuroinflammatory score in different degrees of H. pylori gastritis, compared to normal control. H. pylori, Helicobacter pylori.
FIGURE 2
FIGURE 2
MMP‐9 was mainly expressed in macrophages. (A) Significant differentially expressed genes (DEGs) in H. pylori infection; (B) Single cell sequencing identified the cluster of MMP‐9 in macrophages; (C) Immunofluorescence staining of MMP‐9 and CD11b, scale bar 30 µm; (D) Association between MMP‐9 and macrophages; (E) KEGG pathway analysis of DEGs in MMP+ macrophages and MMP9‐macrophages. H. pylori, Helicobacter pylori; MMP‐9, Matrix metalloproteinase‐9.
FIGURE 3
FIGURE 3
H. pylori infection induced apoptosis of SH‐SY5Y through upregulation of MMP9 in macrophages. (A) Expression of MMP‐9 in THP‐1 cells after H. pylori infection; (B) Zymography of supernatant from infected THP‐1 cell, SB‐3CT (20 μM) were added before infection; (C) (D) SH‐SY5Y cells were treated with supernatant from infected THP‐1 cells, cell viability of SH‐SY5Y cells was measured by AnnexinV‐7AAD staining; (E) Full‐thickness Immunofluorescence staining of PGP9.5 and CD68, M:Mucosa, MM: Muscularis Mucosa,SM: Submucosa, MP: Muscularis Propria, scale bar (left) 500 µm, scale bar (right) 20 µm. H. pylori, Helicobacter pylori; MMP‐9, Matrix metalloproteinase‐9.
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