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. 2023 Oct 13:14:1272393.
doi: 10.3389/fimmu.2023.1272393. eCollection 2023.

Transcriptional responses of liver and spleen in Lota lota to polyriboinosinic polyribocytidylic acid

Fangrui Lou  1 , Yuan Zhang  2 , Anle Xu  3 , Tianxiang Gao  3
Affiliations

Transcriptional responses of liver and spleen in Lota lota to polyriboinosinic polyribocytidylic acid

Fangrui Lou et al. Front Immunol. .

Abstract

Introduction: The cultured Lota lota can meet the market demand in the context of the decline of wild resources, but the disease in the high-density culture process also deserves attention. Therefore, understanding the immune regulation mechanisms of L. lota will be the basis for obtaining high benefits in artificial culture.

Methods: To explore the viral response mechanism of L. lota, RNA-seq was applied to identify the transcriptomic changes of the liver and spleen in L. lota by poly (I:C) stress.

Results: The DEGs (liver: 2186 to 3123; spleen 1542 to 2622) and up-regulated genes (liver: 1231 to 1776; spleen 769 to 1502) in the liver and spleen increased with the prolongation (12h to 48h) of poly (I:C)-stimulation time. This means L. lota needs to mobilize more functional genes in response to longer periods of poly (I:C)-stimulation. Despite the responses of L. lota to poly (I:C) showed tissue-specificity, we hypothesized that both liver and spleen of L. lota can respond to poly (I:C) challenge may be through promoting apoptosis of DNA-damaged cells, increasing the activity of immune-enhancing enzymes, and increasing energy supply based on DEGs annotation information.

Conclusions: Our results demonstrate the transcriptional responses of L. lota to poly (I:C)-stimulation, and these data provide the first resource on the genetic regulation mechanisms of L. lota against viruses. Furthermore, the present study can provide basic information for the prevention of viral diseases in L. lota artificial culture process.

Keywords: Lota lota; liver; poly (I:C); spleen; transcriptome; viral response mechanism.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
The number of genes successfully matched to sequences in the AnimalTFDB, GO, KEGG, KOG, NR, Pfam, Swiss-Prot, and TrEMBL databases, respectively.
Figure 2
Figure 2
The poly (I:C)-stimulated DEGs in liver (A) and spleen (B) of L. lota.
Figure 3
Figure 3
The clustering relationship of DEG expression levels of four experimental groups according to the FPKM.
Figure 4
Figure 4
The common DEGs among four experimental pairs.
Figure 5
Figure 5
The top 30 significantly enriched GO terms of the up- and down regulated genes in the liver and spleen. The vertical coordinate represents the enriched GO terms, and the horizontal coordinate represents the number of DEGs in GO terms. Different colors are used to distinguish biological processes, cellular component, and molecular function. "*" represents the significance of GO terms.
Figure 6
Figure 6
The top 20 significantly enriched KEGG pathways of the up- and down regulated genes in the liver and spleen. The vertical coordinate represents the enriched KEGG pathways, and the horizontal coordinate represents the Rich factor. The size of the dots represents the number of DEGs in KEGG pathways, and different colors of dots represent the different q values.3.5 Validation of immune-related gene expression levels.
Figure 7
Figure 7
Expression levels of immune-related genes obtained based on RNA-seq and qRT-PCR. Asterisks indicate statistical significance (*p < 0.05) or extremely statistical significance (**p < 0.01).
See this image and copyright information in PMC

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