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. 2023 Oct 12:14:1274346.
doi: 10.3389/fmicb.2023.1274346. eCollection 2023.

Isolation and identification of antagonistic Bacillus amyloliquefaciens HSE-12 and its effects on peanut growth and rhizosphere microbial community

Huying Li  1   2 , Chaohui Li  1 , Xin Song  1 , Jintai Li  1 , Pengcheng Zhang  1 , Fengxia Sun  1   3 , Zhigang Geng  4 , Xunli Liu  1   3
Affiliations

Isolation and identification of antagonistic Bacillus amyloliquefaciens HSE-12 and its effects on peanut growth and rhizosphere microbial community

Huying Li et al. Front Microbiol. .

Abstract

The HSE-12 strain isolated from peanut rhizosphere soil was identified as Bacillus amyloliquefaciens by observation of phenotypic characteristics, physiological and biochemical tests, 16S rDNA and gyrB gene sequencing. In vitro experiments showed that the strain possessed biocontrol activity against a variety of pathogens including Sclerotium rolfsii. The strain has the ability to produce hydrolytic enzymes, as well as volatile organic compounds with antagonistic and probiotic effects such as ethyleneglycol and 2,3-butanediol. In addition, HSE-12 showed potassium solubilizing (10.54 ± 0.19 mg/L), phosphorus solubilization (168.34 ± 8.06 mg/L) and nitrogen fixation (17.35 ± 2.34 mg/g) abilities, and was able to secrete siderophores [(Ar-A)/Ar × 100%: 56%] which promoted plant growth. After inoculating peanut with HSE-12, the available phosphorus content in rhizosphere soil increased by 27%, urease activity increased by 43%, catalase activity increased by 70% and sucrase activity increased by 50% (p < 0.05). The dry weight, fresh weight and the height of the first pair of lateral branches of peanuts increased by 24.7, 41.9, and 36.4%, respectively, compared with uninoculated peanuts. In addition, compared with the blank control, it increased the diversity and richness of peanut rhizosphere bacteria and changed the community structure of bacteria and fungi. The relative abundance of beneficial microorganisms such as Sphingomonas, Arthrobacter, RB41, and Micromonospora in rhizosphere soil was increased, while the relative abundance of pathogenic microorganisms such as Aspergillus, Neocosmospora, and Rhizoctonia was decreased.

Keywords: Bacillus amyloliquefaciens; biocontrol; microbial community; plant growth-promoting rhizobacteria; volatile compounds.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
In vitro antagonism of strain HSE-12 against plant pathogens.
Figure 2
Figure 2
Neighbor-joining evolutionary tree constructed from 16S rDNA (A) and gyrB (B) sequence for strain HSE-12. Bootstrap values are shown on the nodes.
Figure 3
Figure 3
Inhibitory effect of VOCs produced by HSE-12 on Sclerotium rolfsii: (A) control treatment; (B) HSE-12 treatment.
Figure 4
Figure 4
Relative abundance of bacterial communities at the phylum level (A) and genus level (B) in peanut rhizosphere soils from HSE-12 and control treatments.
Figure 5
Figure 5
Relative abundance of fungi communities at the phylum level (A) and genus level (B) in peanut rhizosphere soils from HSE-12 and control treatments.
Figure 6
Figure 6
PcoA analysis of bacterial (A) and fungi (B) communities in peanut rhizosphere soil for HSE-12 treatment and control treatment.
Figure 7
Figure 7
Functional prediction of bacterial communities in peanut rhizosphere soil from HSE-12 and control treatments.
Figure 8
Figure 8
Functional prediction of fungi communities in peanut rhizosphere soil from HSE-12 and control treatments.
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