Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Nov-Dec;37(6):2433-2436.
doi: 10.21873/invivo.13348.

A Novel Monochromatic Light to Detect Target Specimens Within Endoscopic Ultrasonography-guided Fine-needle Aspiration Biopsy Samples

Kazuya Matsumoto  1   2 , Masaru Ueki  3 , Kunio Ichino  4 , Takeshi Tsuka  5 , Kazutake Uehara  3 , Yohei Takeda  6 , Takumi Onoyama  6 , Soichiro Kawata  6 , Hiroki Kurumi  6 , Hiroki Koda  6 , Taro Yamashita  6 , Yoshiharu Okamoto  5 , Naoyuki Yamaguchi  7 , Shigeo Sasaki  8 , Hajime Isomoto  6
Affiliations

A Novel Monochromatic Light to Detect Target Specimens Within Endoscopic Ultrasonography-guided Fine-needle Aspiration Biopsy Samples

Kazuya Matsumoto et al. In Vivo. 2023 Nov-Dec.

Abstract

Background/aim: Endoscopic ultrasonography (EUS)-guided fine-needle aspiration biopsy (EUS-FNB) enhances the diagnostic capabilities of EUS by providing additional pathological samples. However, detecting the target specimens within the collected samples can be challenging. The aim of this study was to determine the optimal wavelength of light for detection of target specimens within EUS-FNB samples in an animal experiment.

Materials and methods: EUS-FNB pancreatic tissue samples were collected from a male beagle (weight, 10 kg), and the samples were illuminated with monochromatic light ranging from 430 to 700 nm in 5-nm intervals. The intensities of the target specimen and blood samples were analyzed using the densitometry of the images obtained through irradiation.

Results: We found that transmitted monochromatic light of 605 nm most vividly enhanced the contrast between the target specimens and blood in the samples in the impression of appearance.

Conclusion: Thus, microscopical observations under transmitted light of 605 nm are optimal for target tissue identification within EUS-FNB samples.

Keywords: Endoscopic ultrasound-guided fine needle aspiration; light microscopy; pancreas; rapid on-site evaluation.

PubMed Disclaimer

Conflict of interest statement

The Authors declare no conflicts of interest in relation to this study.

Figures

Figure 1
Figure 1. Consideration for a novel monochromatic light to detect target specimens within endoscopic ultrasonography-guided fine-needle aspiration biopsy samples. (A) Visual inspection and discrimination of the target specimen in the samples. The samples are readily detectable at 595-635 nm. (B) The target specimen, detected by transmitted light of 605 nm, is confirmed as pancreatic tissue in microscopic analysis. (C) The contrast peaks at 600-605 nm because the absorbance of the tissue component increases, as shown in the comparative contrast analysis between the target specimen and blood, by using densitometry. Contrast analysis is performed on the target specimen and the blood in the sample. The contrast between the target specimens and blood peaks at 600-605 nm.
See this image and copyright information in PMC

References

    1. Hewitt MJ, McPhail MJ, Possamai L, Dhar A, Vlavianos P, Monahan KJ. EUS-guided FNA for diagnosis of solid pancreatic neoplasms: a meta-analysis. Gastrointest Endosc. 2012;75(2):319–331. doi: 10.1016/j.gie.2011.08.049. - DOI - PubMed
    1. Matsumoto K, Yamao K, Ohashi K, Watanabe Y, Sawaki A, Nakamura T, Matsuura A, Suzuki T, Fukutomi A, Baba T, Okubo K, Tanaka K, Moriyama I, Shimizu Y. Acute portal vein thrombosis after EUS-guided FNA of pancreatic cancer: case report. Gastrointest Endosc. 2003;57(2):269–271. doi: 10.1067/mge.2003.79. - DOI - PubMed
    1. Hirooka Y, Goto H, Itoh A, Hashimoto S, Niwa K, Ishikawa H, Okada N, Itoh T, Kawashima H. Case of intraductal papillary mucinous tumor in which endosonography-guided fine-needle aspiration biopsy caused dissemination. J Gastroenterol Hepatol. 2003;18(11):1323–1324. doi: 10.1046/j.1440-1746.2003.03040.x. - DOI - PubMed
    1. Matsumoto K, Hara K, Sawaki A, Mizuno N, Hijioka S, Imamura H, Niwa Y, Tajika M, Kawai H, Kondo S, Inaba Y, Yamao K. Ruptured pseudoaneurysm of the splenic artery complicating endoscopic ultrasound-guided fine-needle aspiration biopsy for pancreatic cancer. Endoscopy. 2010;42 Suppl 42(S 02):E27–E28. doi: 10.1055/s-0029-1215323. - DOI - PubMed
    1. Wang KX, Ben QW, Jin ZD, Du YQ, Zou DW, Liao Z, Li ZS. Assessment of morbidity and mortality associated with EUS-guided FNA: a systematic review. Gastrointest Endosc. 2011;73(2):283–290. doi: 10.1016/j.gie.2010.10.045. - DOI - PubMed