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. 2024 Jun 1;19(6):1179-1180.
doi: 10.4103/1673-5374.385862. Epub 2023 Sep 22.

Imaging mass spectrometry: a molecular microscope for studying the role of lipids in Parkinson's disease

Vinata Vedam-Mai  1 , Jacob M Samuel  2 , Boone M Prentice  2
Affiliations

Imaging mass spectrometry: a molecular microscope for studying the role of lipids in Parkinson's disease

Vinata Vedam-Mai et al. Neural Regen Res. .
No abstract available

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Figures

Figure 1
Figure 1
Interplay between α-syn and lipids in Lewy body dementia. α-Syn and lipid imbalance influence each other. α-Syn dyshomeostasis triggers lipid imbalance, which results in the accumulation of free fatty acids, Di and triacylglycerols, that cause oxidative and cellular stress and disease progression. Created with BioRender.com. α-syn: α-Synuclein; DAG: diacylglycerol; PD: Parkinson’s disease; TAG: triacylglycerol.
Figure 2
Figure 2
Imaging mass spectrometry workflow. Created with BioRender.com. Unpublished data. MALDI: Matrix-assisted laser desorption/ionization.
Figure 3
Figure 3
Representative images of thionine-stained brain sections and oval areas. (Top) Representative images of thionine-stained brain sections of control (A), Alzheimer’s disease (AD) I–II (Braak’s stage) (B), AD III–IV (C), AD V–VI (D). (Bottom) The black oval areas are the regions of interest (ROI) selected for measurement in gray matter and the red oval areas are the ROI in white matter. Scale bar: 1 cm. The image shows the distribution of some representative lipid species that were found modified in gray or white matter areas of the frontal cortex from human brain tissue (controls and AD patients at three different stages of the disease). The spectra were recorded with 150 μm spacing. Ten shots were accumulated at each point with the spectrometer working in positive ion mode. Laser power was set at 15–20 μJ. Scale bar: 3 mm. Color scale indicates total ion chromatogram (TIC) normalized intensity. Reprinted with permission from Gónzalez de San Román et al. (2017).
See this image and copyright information in PMC

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