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. 2023 Dec;50(12):10083-10095.
doi: 10.1007/s11033-023-08840-4. Epub 2023 Nov 1.

Delta of Exopalaemon carinicauda: molecular characterization, expression in different tissues and developmental stages, and its SNPs association analysis with development

Xiaofang Lai  1   2   3   4 , Fanyue Ji  5 , Feifan Yu  5 , Hao Chen  5 , Shanrui Shen  5 , Huan Gao  5   6   7   8
Affiliations

Delta of Exopalaemon carinicauda: molecular characterization, expression in different tissues and developmental stages, and its SNPs association analysis with development

Xiaofang Lai et al. Mol Biol Rep. 2023 Dec.

Abstract

Background: The Notch signaling pathway plays a significant role in the gene regulatory network of development of vertebrate and invertebrate. However, as a ligand for the Notch signaling pathway, the mechanism of Delta in the development of Exopalaemon carinicauda is still unclear.

Methods and results: The Delta's molecular characteristics, tissue distribution and their association with development in E. carinicauda were studied by RACE (rapid amplification of cDNA end), qRT-PCR (quantitative Real-time PCR) and SNP (single nucleotide polymorphism), respectively. The delta in E. carinicauda had a full-length cDNA of 2807 bp and its Delta of 808 amino-acid residue had the highest identity with the Delta of Homarus americanus (identity = 76.63%). Delta had the highest expression in the ovary, and its expression varied with different stages of embryonic, larval, and ovarian development. After delta RNA interference (with a highest interference efficiency of 66% at 24 h), the expression of Notch signaling pathway genes and developmental related genes was significantly reduced, and the ovarian development was significantly delayed. Further study found that there were 4 SNPs (ds1-4) in delta cDNA, of which two (ds2 T1521G caused a mutation Asn422Lys and ds3 G1674A caused a mutation Tyr473Cys in the EGF-like domain) were associated with the development of E. carinicauda. The Gonadosomatic Index (GSI) of the ds2 TT genotypes was 37.28% and 134.60% higher than E. carinicauda of GT and GG genotype respectively (P < 0.05).

Conclusion: Our research indicated that delta was involved in the development of E. carinicauda and provided new insights for molecular breeding with SNP markers in E. carinicauda.

Keywords: Association between SNPs and development; Cloning and expression; Delta; Exopalaemon carinicauda; RNA interference.

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References

    1. Pei Z, Baker N (2008) Competition between delta and the Abruptex domain of Notch. BMC Dev Biol 8(1):4. https://doi.org/10.1186/1471-213X-8-4 - DOI - PubMed - PMC
    1. Richards GS, Degnan BM (2012) The expression of delta ligands in the sponge Amphimedon queenslandica suggests an ancient role for notch signaling in metazoan development. EvoDevo 3:15. https://doi.org/10.1186/2041-9139-3-15 - DOI - PubMed - PMC
    1. Lai X, Chen H, Mi X, Shen S, Gao H (2023) Molecular characterization and developmental function of jagged-1 in Exopalaemon carinicauda (Decapoda, Caridea, Palaemonidae). Crustaceana 96(7):683–702. https://doi.org/10.1163/15685403-bja10301 - DOI
    1. Liao B, Oates CA (2016) Delta-Notch signalling in segmentation. Arthropod Struct Dev 46(3):429–447. https://doi.org/10.1016/j.asd.2016.11.007 - DOI - PubMed
    1. Medwig-Kinney TN, Sirota SS, Gibney TV, Pani AM, Matus DQ (2022) An in vivo toolkit to visualize endogenous LAG-2/delta and LIN-12/Notch signaling in C. elegans. Micro Publ Biol 2022:602. https://doi.org/10.17912/micropub.biology.000602 - DOI

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