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. 2023 Dec;50(12):10147-10155.
doi: 10.1007/s11033-023-08875-7. Epub 2023 Nov 3.

Effect of vitamin D on cardiac hypertrophy in D-galactose-induced aging model through cardiac mitophagy

Siamak Shahidi #  1   2   3 , Khadijeh Ramezani-Aliakbari #  4 , Alireza Komaki  2   3 , Iraj Salehi  2   3 , Sayedpayam Hashemi  5 , Sara Soleimani Asl  6 , Parisa Habibi  1   2 , Fatemeh Ramezani-Aliakbari  7   8
Affiliations

Effect of vitamin D on cardiac hypertrophy in D-galactose-induced aging model through cardiac mitophagy

Siamak Shahidi et al. Mol Biol Rep. 2023 Dec.

Abstract

Background: Cardiac apoptosis plays a key role in increased morbidity associated with aging-induced-cardiac disorder. Mitochondria play an important role in cardiac apoptosis, and dynamin-related protein 1 (Drp1), as a main mediator of mitochondrial fission, can trigger the mitophagy process to sustain the mitochondrial quality. The present study was done to determine the effect of vitamin D (VitD) treatment on cardiac hypertrophy through mitophagy regulation in aged animals induced by D-galactose (D-GAL).

Methods and results: Male Wistar rats were randomly divided into four groups: control, D-GAL (aging group), D-GAL co-injected with VitD (D-GAL ± VitD), and D-GAL plus ethanol (D-GAL ± Ethanol). Aging was induced by an intraperitoneal (i.p.) administration of D-GAL at 150 mg/kg daily for eight weeks and also VitD (400 IU/kg) or ethanol was injected (i.p.) into aging rats. Then, the levels of cardiac mitophagy and cardiac apoptosis were determined by measuring the expression of tensin homologue (PTEN)-induced putative kinase 1 (PINK1), Drp1, Bcl2-Associated X (Bax), and B-cell lymphoma 2 (Bcl2) genes. Aging in rats was associated with a reduction in mitophagy and also an increase in apoptosis of the heart through down-regulation of Drp1, PINK1, and Bcl2 genes and also up-regulation of Bax. However, VitD improved cardiac hypertrophy through cardiac mitophagy in D-GAL-induced aging rats.

Conclusion: VitD can inhibit cardiac hypertrophy by an increase in mitophagy and a decrease in apoptosis in the aging heart. The illustration of the suggested mechanism underlying of Vitamin D in cardiac hypertrophy induced by aging.

Keywords: Aging; Apoptosis; Drp1; Mitophagy; PINK1; Vitamin D.

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References

    1. Tepp K et al (2016) Bioenergetics of the aging heart and skeletal muscles: modern concepts and controversies. Ageing Res Rev 28:1–14 - PubMed - DOI
    1. Chadda KR et al (2018) Ageing, the autonomic nervous system and arrhythmia: from brain to heart. Ageing Res Rev 48:40–50 - PubMed - DOI
    1. Thai PN et al (2019) Mitochondrial quality control in aging and heart failure: influence of ketone bodies and mitofusin-stabilizing peptides. Front Physiol 10:382 - PubMed - PMC - DOI
    1. Wang K et al (2013) Thioredoxin reductase was nitrated in the aging heart after myocardial ischemia/reperfusion. Rejuvenation Res 16(5):377–385 - PubMed - PMC - DOI
    1. Kwak H-B (2013) Effects of aging and exercise training on apoptosis in the heart. J Exerc Rehabil 9(2):212 - PubMed - PMC - DOI

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