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. 2023 Nov 3;18(11):e0288147.
doi: 10.1371/journal.pone.0288147. eCollection 2023.

Serum cytokines MCP-1 and GCS-F as potential biomarkers in pediatric inflammatory bowel disease

Andrea Ott  1 , Erol Tutdibi  1 , Sybelle Goedicke-Fritz  1 , Jakob Schöpe  2 , Michael Zemlin  1 , Nasenien Nourkami-Tutdibi  1
Affiliations

Serum cytokines MCP-1 and GCS-F as potential biomarkers in pediatric inflammatory bowel disease

Andrea Ott et al. PLoS One. .

Abstract

Background: Inflammatory bowel diseases (IBDs) with the subtypes ulcerative colitis (UC) and Crohn disease (CD), are chronic autoimmune inflammatory disorders of the gastrointestinal tract. Cytokines are associated with the development and progression in pediatric IBD. We measured cytokine levels in pediatric IBD patients to assess their potential function as biomarkers in disease assessment.

Method: In this prospective cohort study, we enrolled 33 children with IBD. All patients were in stable remission for 3 months on enrollment. Patients who developed a relapse within six months after enrollment were classified as relapsers. Blood sampling was performed at enrolment and for relapsers in relapse and post-relapse. Serum concentrations of 14 cytokines, chemokines and growth factors (IL-1α, IL-1β, IL-6, IL-12p40, IP-10, TNF-α, IFN-γ, IL-10, IL-8, MIP-1α, MCP-1, MCP-3, G-CSF, GM-CSF) were measured simultaneously using multiplex bead-based sandwich immunoassay on Luminex 100 system.

Results: MCP-1 was significantly higher in CD patients compared to UC patients at each disease stage: stable remission (P<0.048), unstable remission (P<0.013), relapse (P<0.026) and post-relapse (P<0.024). G-CSF was significantly increased in UC patients developing a relapse and in post-relapse stage compared to UC patients in remission (P<0.02 and p<0.03, respectively).

Conclusion: MCP-1 showed potential as a diagnostic biomarker in CD patients independent of disease activity as it was able to discriminate between subtypes of pediatric IBD. In UC patients, G-CSF was significantly elevated in relapsers indicating its use and role as a potential prognostic biomarker.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Study design with classification of IBD patients and samplings according to disease course after enrolment.
Fig 2
Fig 2
a-c: Serum concentrations (pg/ml) of MCP-1 and G-CSF in CD and UC patients. a) compares MCP-1 and G-CSF for CD versus UC, b) compares MCP-1 values for different time points in CD and UC patients and c) compares G-CSF levels for different time points in CD and UC. Data are presented as box plots by the method of Tukey. Comparison of cytokine values of CD with UC patients and of stable remission with relapsing group were performed using unpaired Mann-Whitney U-test. Cytokine concentrations within relapsing group (unstable remission to relapse and post-relapse) were analyzed by paired Wilcoxon test. *P<0.05 was considered as statistically significant.
See this image and copyright information in PMC

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