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. 2023 Sep 29:33:100401.
doi: 10.1016/j.jctube.2023.100401. eCollection 2023 Dec.

Histopathological features in the clinical specimens with tuberculosis diagnosis by BACTEC MGIT 960 culture

Nguyen Kim Cuong  1   2 , Dang Van Thanh  3 , Dinh Van Luong  4 , Nguyen Manh The  1 , Truong Duc Thai  1 , Anh Tran Thi Tuan  5 , Doan Thu Ha  6 , Vu Quoc Dat  3   7
Affiliations

Histopathological features in the clinical specimens with tuberculosis diagnosis by BACTEC MGIT 960 culture

Nguyen Kim Cuong et al. J Clin Tuberc Other Mycobact Dis. .

Abstract

Diagnosis of extrapulmonary tuberculosis remains challenging in lower-middle income countries with high burden of tuberculosis (TB). This study aims to describe the histological characteristics in biopsy samples from patients with confirmed TB. This is a retrospective study of clinical biopsy specimens with positive liquid medium culture for Mycobacterium tuberculosis and histopathological examination in the National Lung Hospital in Vietnam. Among 1045 biopsy specimens with mycobacteria culture, the overall rate of growth of Mycobacteria tuberculosis in culture was 20.7% (216/1045). The positivity rates of MIGT culture among surgical biopsy specimens were 75% in bone specimen, followed by vertebral specimens (51.3%), and joint specimens (26.4%). For specimens obtained by the fine needle aspiration, the positivity rates of MIGT culture were 26.3% in lymph node and 25.3% in pleural specimen. Among specimens with culture confirmation of TB, the most common histopathoglogical suggestive finding of TB was the presence of epithelioid cell (83.3%), Langhans giant cells (75.9%), and caseous necrosis (75.5%). The high proportion of histological features suggestive of TB among the TB culture confirmed biopsy samples support for further evaluation of histological examination and its combination with other recommended rapid molecular assays in specimens with suspicion of TB.

Keywords: Extrapulmonary tuberculosis; Histopathology; MGIT culture; Mycobacterium tuberculosis.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Flowchart of sample collection.
Fig. 2
Fig. 2
Percentage of MTB positive and negative culture results by sample locations and methods of biopsy.
Fig. 3
Fig. 3
Number of MTB culture confirmed specimens with histological suggestive of tuberculosis.
See this image and copyright information in PMC

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