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Comment
. 2024 Feb;34(2):99-100.
doi: 10.1038/s41422-023-00894-0.

Fanzors: Striking expansion of RNA-guided endonucleases to eukaryotes

Hui Yang  1 , Dinshaw J Patel  2
Affiliations
Comment

Fanzors: Striking expansion of RNA-guided endonucleases to eukaryotes

Hui Yang et al. Cell Res. 2024 Feb.
No abstract available

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Figures

Fig. 1
Fig. 1. Structural and mechanistic comparison of Fanzor with Cas12a and TnpB.
a, e, i Schematic diagrams of the CRISPR-Cas12 (a), IS200/IS605 transposon (e), and Fanzor (i) loci. The potential RuvC domains are indicated in green. The CRISPR repeats are represented as light blue rectangles and spacers are represented as differently colored diamonds (blue and orange) (a). The LE and RE elements of the transposon, the guide elements and ωRNA are indicated (e, i). b, f, j Domain organization of Acidamanococcus sp. AsCas12a (b), Deinococcus radiodurans ISDra2 TnpB (f), and Spizellomyces punctatus SpuFz1 (j). c, g, k Structural comparisons among AsCas12a (c), ISDra2 TnpB (g), and SpuFz1 (k) in complex with the corresponding crRNA/ωRNA and target dsDNA. The structures are aligned based on the guide:target heteroduplex. d, h, l Proposed DNA recognition and cleavage mechanisms. The REC and NUC lobes are colored in light yellow and silver, respectively. The active sites of RuvC domains are indicated by black scissors. The region in stem2 of SpuFz1 ωRNA removed for optimization is indicated by a dashed box (l). TS, target DNA strand. NTS, non-target DNA strand.
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