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. 2023 Aug 25;11(4):601-613.
doi: 10.5599/admet.1897. eCollection 2023.

Evaluation of curcumin-loaded chitosan nanoparticles for wound healing activity

Smita Kumbhar  1 , Rupali Khairate  1 , Manish Bhatia  2 , Prafulla Choudhari  2 , Vinod Gaikwad  3
Affiliations

Evaluation of curcumin-loaded chitosan nanoparticles for wound healing activity

Smita Kumbhar et al. ADMET DMPK. .

Abstract

Background and purpose: Wound healing is a biological process that can be difficult to manage clinically. In skin wound healing, the interaction of many cells, growth factors, and cytokines reveals an outstanding biological function mechanism. Wound healing that occurs naturally restores tissue integrity, however, it is usually restricted to wound repair. Curcumin synthesised in a chitosan matrix can be used to heal skin sores.

Experimental approach: The ionotropic gelation procedure required crosslinking chitosan with a tripolyphosphate (TPP) crosslinker to generate curcumin nanoparticles encapsulated in chitosan.

Key results: The nanoparticles were between 200 and 400 nm in size, with a strong positive surface charge and good entrapment efficacy, according to SEM and TEM investigations. Curcumin and chitosan compatibility was investigated using FTIR spectroscopy. All batches showed consistent drug release, with the F5 batch having the highest curcumin release, at 75% after 16 hours. On L929 cells, scratch assays were utilised to assess wound healing. Wound closure with widths of 59 and 65 mm with curcumin and 45 and 78 mm with curcumin-loaded chitosan nanoparticles was seen after 24 and 48 hours of examination.

Conclusions: According to the findings, prepared curcumin chitosan nanoparticles are beneficial in healing skin damage.

Keywords: Skin regeneration; chitosan; ionotropic gelation; nanoparticles; topical administration; turmeric.

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Conflict of interest statement

Conflict of interest: The authors report no conflicts of interest in this work.

Figures

Figure 1.
Figure 1.
Graphical representation of ionotropic gelation method
Figure 2.
Figure 2.
SEM images of curcumin loaded chitosan nanoparticles
Figure 3.
Figure 3.
TEM image of curcumin-loaded chitosan nanoparticles
Figure 4.
Figure 4.
Zeta potential of curcumin-loaded chitosan nanoparticles
Figure 5.
Figure 5.
FTIR spectra of curcumin-loaded chitosan nanoparticle
Figure 6.
Figure 6.
Overlay of FTIR spectra of curcumin, chitosan and formulated nanoparticles
Figure 7.
Figure 7.
DSC spectra of chitosan
Figure 8.
Figure 8.
DSC spectra of curcumin-loaded chitosan nanoparticles
Figure 9.
Figure 9.
Drug release profile of curcumin-loaded chitosan nanoparticles
Figure 10.
Figure 10.
Cell migration analysis of untreated cells
Figure 11.
Figure 11.
Cell migration analysis of standard cipladine (5 μg/ml)
Figure 12.
Figure 12.
Cell migration analysis of curcumin (100 μg/ml)
Figure 13.
Figure 13.
Cell migration analysis of curcumin-loaded chitosan nanoparticles (100 μg/ml)
See this image and copyright information in PMC

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