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. 2023 Oct;15(5):616-624.
doi: 10.18502/ijm.v15i5.13867.

Characterization of integrons, extended spectrum beta lactamases and genetic diversity among uropathogenic Escherichia coli isolates from Kerman, south east of Iran

Samane Mohebi  1 , Zahra Golestani-Hotkani  2 , Maryam Foulad-Pour  3 , Peivand Nazeri  3 , Fahimeh Mohseni  4 , Zahra Hashemizadeh  1 , Zahra Moghani-Bashi  5 , Naser Niksefat  4 , Sanaz Rastegar  6 , Maryam Khajedadian  4 , Zahra Lotfian  4 , Hossein Hosseini-Nave  6
Affiliations

Characterization of integrons, extended spectrum beta lactamases and genetic diversity among uropathogenic Escherichia coli isolates from Kerman, south east of Iran

Samane Mohebi et al. Iran J Microbiol. 2023 Oct.

Abstract

Background and objectives: The study aimed to investigate the distribution of genes encoding integrons, extended spectrum beta-lactamase (ESBL) in E. coli isolated from UTIs, as well as the genetic diversity among the isolates.

Materials and methods: E. coli isolates were recovered from the patients with UTI in Kerman Iran. Antibiotic susceptibility was done according to CLSI guidelines. The presence of ESBL genes and integrons was evaluated using PCR. PCR and sequencing were applied for the evaluation of cassette content of integrons. Genotyping of the isolates was performed by multiple-locus variable-number tandem repeat analysis (MLVA).

Results: Imipenem was the most effective antibiotic, while the highest resistance was observed to streptomycin. In total 40.2% of isolates were ESBL producers. Of 69 integron-positive isolates, 59 only had class I integrons, 4 only had class II integrons and 6 had both types. The most common gene cassette found within class I integrons was dfrA17-aadA5 (n=27). The E. coli isolates were divided into 16 MLVA clusters.

Conclusion: The current study demonstrated the simultaneous presence of class I integrons and ESBLs involved in the resistance of UPEC isolates to antibacterial agents. Our finding also revealed that the E. coli isolates belonged to diverse clones.

Keywords: Drug resistance; Extended spectrum beta-lactamase; Integrons; Tandem repeat sequences; Uropathogenic Escherichia coli.

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Figures

Fig. 1.
Fig. 1.
UPGMA dendrogram for the 102 UPEC isolates based on seven VNTR loci. Clusters were defined using a similarity cut-off value of 50%, as indicated by the vertical line. The assigned clusters are represented by letters. Strain numbers, integron, and ESBL genes of these isolates are also indicated (+, positive and −, negative).
Fig. 2.
Fig. 2.
An Example of Gel Electrophoresis of VNTR Products. The image shows how the number of repeats can be directly deduced by manual reading. The numbers above the PCR amplicons represent the repeat numbers of ms32 locus. Lanes M, DNA markers
See this image and copyright information in PMC

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